Quantitative T 2* assessment of acute and chronic myocardial ischemia/reperfusion injury in mice

E.N.E. Aguor, F. Arslan, C.W.A. van de Kolk, M.G.J. Nederhoff, P.A.F.M. Doevendans, C.J.A. van Echteld, G. Pasterkamp, G.J. Strijkers

Research output: Contribution to journalArticleAcademicpeer-review


OBJECT: Imaging of myocardial infarct composition is essential to assess efficacy of emerging therapeutics. T (2) (*) mapping has the potential to image myocardial hemorrhage and fibrosis by virtue of its short T (2) (*) . We aimed to quantify T (2) (*) in acute and chronic myocardial ischemia/reperfusion (I/R) injury in mice.

MATERIALS AND METHODS: I/R-injury was induced in C57BL/6 mice (n = 9). Sham-operated mice (n = 8) served as controls. MRI was performed at baseline, and 1, 7 and 28 days after surgery. MRI at 9.4 T consisted of Cine, T (2) (*) mapping and late-gadolinium-enhancement (LGE). Mice (n = 6) were histologically assessed for hemorrhage and collagen in the fibrotic scar.

RESULTS: Baseline T (2) (*) values were 17.1 ± 2.0 ms. At day 1, LGE displayed a homogeneous infarct enhancement. T (2) (*) in infarct (12.0 ± 1.1 ms) and remote myocardium (13.9 ± 0.8 ms) was lower than at baseline. On days 7 and 28, LGE was heterogeneous. T (2) (*) in the infarct decreased to 7.9 ± 0.7 and 6.4 ± 0.7 ms, whereas T (2) (*) values in the remote myocardium were 14.2 ± 1.1 and 15.6 ± 1.0 ms. Histology revealed deposition of iron and collagen in parallel with decreased T (2) (*) .

CONCLUSION: T (2) (*) values are dynamic during infarct development and decrease significantly during scar maturation. In the acute phase, T (2) (*) values in infarcted myocardium differ significantly from those in the chronic phase. T (2) (*) mapping was able to confirm the presence of a chronic infarction in cases where LGE was inconclusive. Hence, T (2) (*) may be used to discriminate between acute and chronic infarctions.

Original languageEnglish
Pages (from-to)369-379
Number of pages11
JournalMagma - Magnetic Resonance Materials In Physics Biology And Medicine
Issue number5
Publication statusPublished - 2012


  • Acute Disease
  • Animals
  • Collagen
  • Disease Models, Animal
  • Fibrosis
  • Heart Ventricles
  • Hemorrhage
  • Iron
  • Magnetic Resonance Imaging, Cine
  • Mice
  • Mice, Inbred C57BL
  • Myocardial Infarction
  • Myocardial Reperfusion Injury
  • Myocardium
  • Time Factors
  • Journal Article
  • Research Support, Non-U.S. Gov't


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