Quantitative T 2* assessment of acute and chronic myocardial ischemia/reperfusion injury in mice

E.N.E. Aguor; F. Arslan; C.W.A. van de Kolk; M.G.J. Nederhoff; P.A.F.M. Doevendans; C.J.A. van Echteld; G. Pasterkamp; G.J. Strijkers

doi:10.1007/s10334-012-0304-0

Quantitative T 2* assessment of acute and chronic myocardial ischemia/reperfusion injury in mice

E.N.E. Aguor, F. Arslan, C.W.A. van de Kolk, M.G.J. Nederhoff, P.A.F.M. Doevendans, C.J.A. van Echteld, G. Pasterkamp, G.J. Strijkers

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

OBJECT: Imaging of myocardial infarct composition is essential to assess efficacy of emerging therapeutics. T (2) (*) mapping has the potential to image myocardial hemorrhage and fibrosis by virtue of its short T (2) (*) . We aimed to quantify T (2) (*) in acute and chronic myocardial ischemia/reperfusion (I/R) injury in mice.

MATERIALS AND METHODS: I/R-injury was induced in C57BL/6 mice (n = 9). Sham-operated mice (n = 8) served as controls. MRI was performed at baseline, and 1, 7 and 28 days after surgery. MRI at 9.4 T consisted of Cine, T (2) (*) mapping and late-gadolinium-enhancement (LGE). Mice (n = 6) were histologically assessed for hemorrhage and collagen in the fibrotic scar.

RESULTS: Baseline T (2) (*) values were 17.1 ± 2.0 ms. At day 1, LGE displayed a homogeneous infarct enhancement. T (2) (*) in infarct (12.0 ± 1.1 ms) and remote myocardium (13.9 ± 0.8 ms) was lower than at baseline. On days 7 and 28, LGE was heterogeneous. T (2) (*) in the infarct decreased to 7.9 ± 0.7 and 6.4 ± 0.7 ms, whereas T (2) (*) values in the remote myocardium were 14.2 ± 1.1 and 15.6 ± 1.0 ms. Histology revealed deposition of iron and collagen in parallel with decreased T (2) (*) .

CONCLUSION: T (2) (*) values are dynamic during infarct development and decrease significantly during scar maturation. In the acute phase, T (2) (*) values in infarcted myocardium differ significantly from those in the chronic phase. T (2) (*) mapping was able to confirm the presence of a chronic infarction in cases where LGE was inconclusive. Hence, T (2) (*) may be used to discriminate between acute and chronic infarctions.

Original language	English
Pages (from-to)	369-379
Number of pages	11
Journal	Magma - Magnetic Resonance Materials In Physics Biology And Medicine
Volume	25
Issue number	5
DOIs	https://doi.org/10.1007/s10334-012-0304-0
Publication status	Published - 2012

Keywords

Acute Disease
Animals
Collagen
Disease Models, Animal
Fibrosis
Heart Ventricles
Hemorrhage
Iron
Magnetic Resonance Imaging, Cine
Mice
Mice, Inbred C57BL
Myocardial Infarction
Myocardial Reperfusion Injury
Myocardium
Time Factors
Journal Article
Research Support, Non-U.S. Gov't

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10.1007/s10334-012-0304-0

http://link.springer.com/article/10.1007%2Fs10334-012-0304-0; http://www.ncbi.nlm.nih.gov/pubmed/22327962

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Aguor, E. N. E., Arslan, F., van de Kolk, C. W. A., Nederhoff, M. G. J., Doevendans, P. A. F. M., van Echteld, C. J. A., Pasterkamp, G., & Strijkers, G. J. (2012). Quantitative T 2* assessment of acute and chronic myocardial ischemia/reperfusion injury in mice. Magma - Magnetic Resonance Materials In Physics Biology And Medicine, 25(5), 369-379. https://doi.org/10.1007/s10334-012-0304-0

@article{8077ef5cb2f14be2be24c174d1ea011a,

title = "Quantitative T 2* assessment of acute and chronic myocardial ischemia/reperfusion injury in mice",

abstract = "OBJECT: Imaging of myocardial infarct composition is essential to assess efficacy of emerging therapeutics. T (2) (*) mapping has the potential to image myocardial hemorrhage and fibrosis by virtue of its short T (2) (*) . We aimed to quantify T (2) (*) in acute and chronic myocardial ischemia/reperfusion (I/R) injury in mice.MATERIALS AND METHODS: I/R-injury was induced in C57BL/6 mice (n = 9). Sham-operated mice (n = 8) served as controls. MRI was performed at baseline, and 1, 7 and 28 days after surgery. MRI at 9.4 T consisted of Cine, T (2) (*) mapping and late-gadolinium-enhancement (LGE). Mice (n = 6) were histologically assessed for hemorrhage and collagen in the fibrotic scar.RESULTS: Baseline T (2) (*) values were 17.1 ± 2.0 ms. At day 1, LGE displayed a homogeneous infarct enhancement. T (2) (*) in infarct (12.0 ± 1.1 ms) and remote myocardium (13.9 ± 0.8 ms) was lower than at baseline. On days 7 and 28, LGE was heterogeneous. T (2) (*) in the infarct decreased to 7.9 ± 0.7 and 6.4 ± 0.7 ms, whereas T (2) (*) values in the remote myocardium were 14.2 ± 1.1 and 15.6 ± 1.0 ms. Histology revealed deposition of iron and collagen in parallel with decreased T (2) (*) .CONCLUSION: T (2) (*) values are dynamic during infarct development and decrease significantly during scar maturation. In the acute phase, T (2) (*) values in infarcted myocardium differ significantly from those in the chronic phase. T (2) (*) mapping was able to confirm the presence of a chronic infarction in cases where LGE was inconclusive. Hence, T (2) (*) may be used to discriminate between acute and chronic infarctions.",

keywords = "Acute Disease, Animals, Collagen, Disease Models, Animal, Fibrosis, Heart Ventricles, Hemorrhage, Iron, Magnetic Resonance Imaging, Cine, Mice, Mice, Inbred C57BL, Myocardial Infarction, Myocardial Reperfusion Injury, Myocardium, Time Factors, Journal Article, Research Support, Non-U.S. Gov't",

author = "E.N.E. Aguor and F. Arslan and {van de Kolk}, C.W.A. and M.G.J. Nederhoff and P.A.F.M. Doevendans and {van Echteld}, C.J.A. and G. Pasterkamp and G.J. Strijkers",

year = "2012",

doi = "10.1007/s10334-012-0304-0",

language = "English",

volume = "25",

pages = "369--379",

journal = "Magma - Magnetic Resonance Materials In Physics Biology And Medicine",

issn = "0968-5243",

publisher = "Springer-Verlag",

number = "5",

}

TY - JOUR

T1 - Quantitative T 2* assessment of acute and chronic myocardial ischemia/reperfusion injury in mice

AU - Aguor, E.N.E.

AU - Arslan, F.

AU - van de Kolk, C.W.A.

AU - Nederhoff, M.G.J.

AU - Doevendans, P.A.F.M.

AU - van Echteld, C.J.A.

AU - Pasterkamp, G.

AU - Strijkers, G.J.

PY - 2012

Y1 - 2012

N2 - OBJECT: Imaging of myocardial infarct composition is essential to assess efficacy of emerging therapeutics. T (2) (*) mapping has the potential to image myocardial hemorrhage and fibrosis by virtue of its short T (2) (*) . We aimed to quantify T (2) (*) in acute and chronic myocardial ischemia/reperfusion (I/R) injury in mice.MATERIALS AND METHODS: I/R-injury was induced in C57BL/6 mice (n = 9). Sham-operated mice (n = 8) served as controls. MRI was performed at baseline, and 1, 7 and 28 days after surgery. MRI at 9.4 T consisted of Cine, T (2) (*) mapping and late-gadolinium-enhancement (LGE). Mice (n = 6) were histologically assessed for hemorrhage and collagen in the fibrotic scar.RESULTS: Baseline T (2) (*) values were 17.1 ± 2.0 ms. At day 1, LGE displayed a homogeneous infarct enhancement. T (2) (*) in infarct (12.0 ± 1.1 ms) and remote myocardium (13.9 ± 0.8 ms) was lower than at baseline. On days 7 and 28, LGE was heterogeneous. T (2) (*) in the infarct decreased to 7.9 ± 0.7 and 6.4 ± 0.7 ms, whereas T (2) (*) values in the remote myocardium were 14.2 ± 1.1 and 15.6 ± 1.0 ms. Histology revealed deposition of iron and collagen in parallel with decreased T (2) (*) .CONCLUSION: T (2) (*) values are dynamic during infarct development and decrease significantly during scar maturation. In the acute phase, T (2) (*) values in infarcted myocardium differ significantly from those in the chronic phase. T (2) (*) mapping was able to confirm the presence of a chronic infarction in cases where LGE was inconclusive. Hence, T (2) (*) may be used to discriminate between acute and chronic infarctions.

AB - OBJECT: Imaging of myocardial infarct composition is essential to assess efficacy of emerging therapeutics. T (2) (*) mapping has the potential to image myocardial hemorrhage and fibrosis by virtue of its short T (2) (*) . We aimed to quantify T (2) (*) in acute and chronic myocardial ischemia/reperfusion (I/R) injury in mice.MATERIALS AND METHODS: I/R-injury was induced in C57BL/6 mice (n = 9). Sham-operated mice (n = 8) served as controls. MRI was performed at baseline, and 1, 7 and 28 days after surgery. MRI at 9.4 T consisted of Cine, T (2) (*) mapping and late-gadolinium-enhancement (LGE). Mice (n = 6) were histologically assessed for hemorrhage and collagen in the fibrotic scar.RESULTS: Baseline T (2) (*) values were 17.1 ± 2.0 ms. At day 1, LGE displayed a homogeneous infarct enhancement. T (2) (*) in infarct (12.0 ± 1.1 ms) and remote myocardium (13.9 ± 0.8 ms) was lower than at baseline. On days 7 and 28, LGE was heterogeneous. T (2) (*) in the infarct decreased to 7.9 ± 0.7 and 6.4 ± 0.7 ms, whereas T (2) (*) values in the remote myocardium were 14.2 ± 1.1 and 15.6 ± 1.0 ms. Histology revealed deposition of iron and collagen in parallel with decreased T (2) (*) .CONCLUSION: T (2) (*) values are dynamic during infarct development and decrease significantly during scar maturation. In the acute phase, T (2) (*) values in infarcted myocardium differ significantly from those in the chronic phase. T (2) (*) mapping was able to confirm the presence of a chronic infarction in cases where LGE was inconclusive. Hence, T (2) (*) may be used to discriminate between acute and chronic infarctions.

KW - Acute Disease

KW - Animals

KW - Collagen

KW - Disease Models, Animal

KW - Fibrosis

KW - Heart Ventricles

KW - Hemorrhage

KW - Iron

KW - Magnetic Resonance Imaging, Cine

KW - Mice

KW - Mice, Inbred C57BL

KW - Myocardial Infarction

KW - Myocardial Reperfusion Injury

KW - Myocardium

KW - Time Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s10334-012-0304-0

DO - 10.1007/s10334-012-0304-0

M3 - Article

C2 - 22327962

SN - 0968-5243

VL - 25

SP - 369

EP - 379

JO - Magma - Magnetic Resonance Materials In Physics Biology And Medicine

JF - Magma - Magnetic Resonance Materials In Physics Biology And Medicine

IS - 5

ER -

Quantitative T 2* assessment of acute and chronic myocardial ischemia/reperfusion injury in mice

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