TY - JOUR
T1 - Quantitative evaluation of T-cell response after specific antigen stimulation in active and latent tuberculosis infection in adults and children
AU - Latorre, Irene
AU - De Souza-Galvão, Malú
AU - Ruiz-Manzano, Juan
AU - Lacoma, Alicia
AU - Prat, Cristina
AU - Fuenzalida, Loreto
AU - Altet, Neus
AU - Ausina, Vicente
AU - Domínguez, Jose
N1 - Funding Information:
This work was supported by a grant from the Sociedad Española de Neumología y Cirugía Torácica (SEPAR)(Barcelona, Spain), the Societat Catalana de Pneumologia (SOCAP) (Barcelona, Spain), the Fundació Catalana de Pneumologia (FUCAP) (Barcelona, Spain), and Instituto de Salud Carlos III (Madrid, Spain) (RETIC RD06/0018). QuantiFERON-TB GOLD In-Tube kits were provided by Inverness Medical Ibérica SAU, Cellestis' distributor in Spain, and T.SPOT-TB was provided by Oxford Immunotec by means of a Material Transfer Agreement.
Funding Information:
The authors thank the nurse staff of the TB Control and Prevention Unit of Barcelona and C. Ramil, L. Haba, M.A. Cuesta, M. Pérez, J. Maldonado, and J.M. Pina for technical assistance and helpful discussions. I. Latorre is Formación de Profesorado Universitario (FPU) predoctoral student and is the recipient of a grant from the “Ministerio de Educación y Ciencia” (Madrid, Spain).
PY - 2009/11
Y1 - 2009/11
N2 - We have evaluated the quantitative T-cell response after specific Mycobacterium tuberculosis antigen stimulation in active tuberculosis (TB) and latent TB infection (LTBI) patients. In adults, the median number of T cells after RD1 antigen stimulation was significantly higher in active TB patients than in LTBI patients. In children, the number of responder T cells against the specific antigens was higher in active TB than in LTBI patients, although the differences were not significant. In summary, in patients with suspected clinical TB, although there is overlapping in the number of responder T cells between both groups, a T-cell count above the described threshold could suggest active TB, especially in patients with a high probability of having active TB and low probability of having LTBI. In addition, the results are consistent with the current evidence that T-cell response may indicate mycobacterial burden and disease activity.
AB - We have evaluated the quantitative T-cell response after specific Mycobacterium tuberculosis antigen stimulation in active tuberculosis (TB) and latent TB infection (LTBI) patients. In adults, the median number of T cells after RD1 antigen stimulation was significantly higher in active TB patients than in LTBI patients. In children, the number of responder T cells against the specific antigens was higher in active TB than in LTBI patients, although the differences were not significant. In summary, in patients with suspected clinical TB, although there is overlapping in the number of responder T cells between both groups, a T-cell count above the described threshold could suggest active TB, especially in patients with a high probability of having active TB and low probability of having LTBI. In addition, the results are consistent with the current evidence that T-cell response may indicate mycobacterial burden and disease activity.
KW - Active TB
KW - Children
KW - IFN-γ tests
KW - LTBI
KW - Mycobacterial burden
UR - http://www.scopus.com/inward/record.url?scp=71749093511&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2009.07.015
DO - 10.1016/j.diagmicrobio.2009.07.015
M3 - Article
C2 - 19822269
AN - SCOPUS:71749093511
SN - 0732-8893
VL - 65
SP - 236
EP - 246
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 3
ER -