Quantitative analysis of phagocytosis of Cryptococcus neoformans by adherent phagocytic cells by fluorescence multi-well plate reader

A. M.E. Walenkamp; J. Scharringa; F. M.N.H. Schramel; F. E.J. Coenjaerts; I. M. Hoepelman

doi:10.1016/S0167-7012(99)00128-1

Quantitative analysis of phagocytosis of Cryptococcus neoformans by adherent phagocytic cells by fluorescence multi-well plate reader

A. M.E. Walenkamp, J. Scharringa, F. M.N.H. Schramel, F. E.J. Coenjaerts^*, I. M. Hoepelman

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

Macrophages and monocytes are adherent phagocytic cells which play an important role in host defence against the yeast-like fungus Cryptococcus neoformans. Before, phagocytosis by adherent phagocytes could only be measured by means of microscopy or by a radioactive assay, which both have obvious disadvantages. We have developed a new, rapid and objective method to measure phagocytosis of C. neoformans by adherent phagocytes (e.g. alveolar macrophages) using a fluorescence multi-well plate reader. This method allows us to discriminate accurately between adherence and internalisation of C. neoformans by macrophages during long term incubation. In addition, the method was used to study the role of the mannose receptor in phagocytosis of the acapsular yeast in the absence of serum by human monocyte-derived macrophages (MDM). (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	39-45
Number of pages	7
Journal	Journal of Microbiological Methods
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/S0167-7012(99)00128-1
Publication status	Published - 1 Mar 2000

Keywords

Adherent cells
Cryptococcus neoformans
Method
Phagocytosis

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10.1016/S0167-7012(99)00128-1

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title = "Quantitative analysis of phagocytosis of Cryptococcus neoformans by adherent phagocytic cells by fluorescence multi-well plate reader",

abstract = "Macrophages and monocytes are adherent phagocytic cells which play an important role in host defence against the yeast-like fungus Cryptococcus neoformans. Before, phagocytosis by adherent phagocytes could only be measured by means of microscopy or by a radioactive assay, which both have obvious disadvantages. We have developed a new, rapid and objective method to measure phagocytosis of C. neoformans by adherent phagocytes (e.g. alveolar macrophages) using a fluorescence multi-well plate reader. This method allows us to discriminate accurately between adherence and internalisation of C. neoformans by macrophages during long term incubation. In addition, the method was used to study the role of the mannose receptor in phagocytosis of the acapsular yeast in the absence of serum by human monocyte-derived macrophages (MDM). (C) 2000 Elsevier Science B.V.",

keywords = "Adherent cells, Cryptococcus neoformans, Method, Phagocytosis",

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T1 - Quantitative analysis of phagocytosis of Cryptococcus neoformans by adherent phagocytic cells by fluorescence multi-well plate reader

AU - Walenkamp, A. M.E.

AU - Scharringa, J.

AU - Schramel, F. M.N.H.

AU - Coenjaerts, F. E.J.

AU - Hoepelman, I. M.

PY - 2000/3/1

Y1 - 2000/3/1

N2 - Macrophages and monocytes are adherent phagocytic cells which play an important role in host defence against the yeast-like fungus Cryptococcus neoformans. Before, phagocytosis by adherent phagocytes could only be measured by means of microscopy or by a radioactive assay, which both have obvious disadvantages. We have developed a new, rapid and objective method to measure phagocytosis of C. neoformans by adherent phagocytes (e.g. alveolar macrophages) using a fluorescence multi-well plate reader. This method allows us to discriminate accurately between adherence and internalisation of C. neoformans by macrophages during long term incubation. In addition, the method was used to study the role of the mannose receptor in phagocytosis of the acapsular yeast in the absence of serum by human monocyte-derived macrophages (MDM). (C) 2000 Elsevier Science B.V.

AB - Macrophages and monocytes are adherent phagocytic cells which play an important role in host defence against the yeast-like fungus Cryptococcus neoformans. Before, phagocytosis by adherent phagocytes could only be measured by means of microscopy or by a radioactive assay, which both have obvious disadvantages. We have developed a new, rapid and objective method to measure phagocytosis of C. neoformans by adherent phagocytes (e.g. alveolar macrophages) using a fluorescence multi-well plate reader. This method allows us to discriminate accurately between adherence and internalisation of C. neoformans by macrophages during long term incubation. In addition, the method was used to study the role of the mannose receptor in phagocytosis of the acapsular yeast in the absence of serum by human monocyte-derived macrophages (MDM). (C) 2000 Elsevier Science B.V.

KW - Adherent cells

KW - Cryptococcus neoformans

KW - Method

KW - Phagocytosis

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U2 - 10.1016/S0167-7012(99)00128-1

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JO - Journal of Microbiological Methods

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ER -

Quantitative analysis of phagocytosis of Cryptococcus neoformans by adherent phagocytic cells by fluorescence multi-well plate reader

Abstract

Keywords

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