Protocol to profile the bioenergetics of organoids using Seahorse

Marlies C. Ludikhuize; Maaike Meerlo; Boudewijn M.T. Burgering; Maria J. Rodríguez Colman

doi:10.1016/j.xpro.2021.100386

Protocol to profile the bioenergetics of organoids using Seahorse

Marlies C. Ludikhuize^*, Maaike Meerlo, Boudewijn M.T. Burgering, Maria J. Rodríguez Colman

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Addressing bioenergetics is key to evaluate the impact of metabolism on the regulation of biological processes and its alteration in disease. Organoids are in vitro grown self-organizing structures derived from healthy and diseased tissue that recapitulate with high fidelity the tissue of origin. Bioenergetics is commonly analyzed by Seahorse XF analysis. However, its application to organoid studies is technically challenging. Here, we share our in-house optimized protocols to examine organoid bioenergetics in response to drugs, gene knockdown, or to characterize the metabolism of specific cell types. For complete details on the use and execution of this protocol, please refer to Ludikhuize et al. (2020).

Original language	English
Article number	100386
Journal	STAR protocols
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2021.100386
Publication status	Published - 19 Mar 2021

Keywords

Metabolism
Organoids
Stem cells

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10.1016/j.xpro.2021.100386Licence: CC BY-NC-ND

1-s2.0-S2666166721000939-mainFinal published version, 3.85 MBLicence: CC BY-NC-ND

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abstract = "Addressing bioenergetics is key to evaluate the impact of metabolism on the regulation of biological processes and its alteration in disease. Organoids are in vitro grown self-organizing structures derived from healthy and diseased tissue that recapitulate with high fidelity the tissue of origin. Bioenergetics is commonly analyzed by Seahorse XF analysis. However, its application to organoid studies is technically challenging. Here, we share our in-house optimized protocols to examine organoid bioenergetics in response to drugs, gene knockdown, or to characterize the metabolism of specific cell types. For complete details on the use and execution of this protocol, please refer to Ludikhuize et al. (2020).",

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note = "Funding Information: This work was financially supported by Dutch Cancer Society ( KWF 2016-I 10471 , KWF 2017-II 11315 ). Publisher Copyright: {\textcopyright} 2021 The Authors",

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Protocol to profile the bioenergetics of organoids using Seahorse

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