TY - JOUR
T1 - Protocol for the Isolation of Intact Chondrons from Healthy and Osteoarthritic Human Articular Cartilage
AU - Uzieliene, Ilona
AU - Denkovskij, Jaroslav
AU - Bernotiene, Eiva
AU - Kalvaityte, Ursule
AU - Vaiciuleviciute, Raminta
AU - Ramos, Yolande F M
AU - Mobasheri, Ali
N1 - Funding Information:
We wish to acknowledge funding from the European Structural and Social Funds through the Research Council of Lithuania (Lietuvos Mokslo Taryba) according to the Programme “Attracting Foreign Researchers for Research Implementation,” Grant No 0.2.2- LMTK-718-02-0022. Y.F.M.R. acknowledges financial support of the Dutch Scientific Research Council NWO/ZonMW VICI scheme (nr 91816631/528), the Leiden University Medical Center for supporting the ongoing RAAK study, and all RAAK study participants and members of the Meulenbelt lab at the LUMC. Author disclosures: I.U., J.D., E.B., U.K., R.V., and Y.F.M.R. have no conflict of interest to disclose. AM has consulted for and received grants from the following companies during the last three years: Abbvie, AchéLaboratórios Farmacêuticos S.A., Ster-ifarma, Genacol, Galapagos, Kolon TissueGene, Pfizer Inc., Pfizer Consumer Health (PCH), GSK Consumer Healthcare, Servier, Bioiberica S.A., and Artialis S.A.
Publisher Copyright:
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021
Y1 - 2021
N2 - Chondrons are the main functional microanatomical units in cartilage, consisting of chondrocytes and the directly surrounding pericellular matrix (PCM). They have attracted attention as a more physiological and biomimetic in vitro model for evaluating chondrocyte function and metabolism as compared to single chondrocytes. Chondrons may be more suitable for in vitro studies than primary chondrocytes that have been isolated without PCM since their in situ and in vivo states remain intact: chondrocytes within their PCM do not undergo the rapid dedifferentiation that proliferating single chondrocytes undergo in culture. Therefore, chondrons may be a better model for studying chondrocyte biology and responses to pro-inflammatory and anti-inflammatory cytokines, growth factors and novel therapeutics. In this chapter, we present a concise and unified protocol for enzymatic isolation of intact chondrons from human articular cartilage and determination of their viability.
AB - Chondrons are the main functional microanatomical units in cartilage, consisting of chondrocytes and the directly surrounding pericellular matrix (PCM). They have attracted attention as a more physiological and biomimetic in vitro model for evaluating chondrocyte function and metabolism as compared to single chondrocytes. Chondrons may be more suitable for in vitro studies than primary chondrocytes that have been isolated without PCM since their in situ and in vivo states remain intact: chondrocytes within their PCM do not undergo the rapid dedifferentiation that proliferating single chondrocytes undergo in culture. Therefore, chondrons may be a better model for studying chondrocyte biology and responses to pro-inflammatory and anti-inflammatory cytokines, growth factors and novel therapeutics. In this chapter, we present a concise and unified protocol for enzymatic isolation of intact chondrons from human articular cartilage and determination of their viability.
KW - Articular cartilage
KW - Chondrocytes
KW - Chondrons
KW - Extracellular matrix (ECM)
KW - Osteoarthritis
KW - Pericellular matrix (PCM)
UR - http://www.scopus.com/inward/record.url?scp=85097899509&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-1119-7_2
DO - 10.1007/978-1-0716-1119-7_2
M3 - Article
C2 - 33315192
SN - 1064-3745
VL - 2245
SP - 13
EP - 22
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -