TY - JOUR
T1 - Protocol for the generation and differentiation of thymic epithelial organoids from adult mouse thymus tissue
AU - Willemsen, Sam
AU - Yanti, Ni Luh Wisma Eka
AU - van Es, Johan H.
AU - Clevers, Hans
AU - Lim, Sangho
N1 - Publisher Copyright:
© 2025 The Author(s)
PY - 2025/6/20
Y1 - 2025/6/20
N2 - Here, we present a protocol for generating long-term expandable thymic epithelial cell (TEC) organoids from adult mouse thymus. We describe the steps for tissue isolation, dissociation, 3D culture matrix embedding, organoid maintenance, and passaging. We then detail procedures for TEC differentiation into cortical and medullary TEC lineages using defined growth factors, and techniques for analyzing organoids, including flow cytometry and qPCR. This protocol provides a valuable tool for studying TEC development and function. For complete details on the use and execution of this protocol, please refer to Lim et al.1
AB - Here, we present a protocol for generating long-term expandable thymic epithelial cell (TEC) organoids from adult mouse thymus. We describe the steps for tissue isolation, dissociation, 3D culture matrix embedding, organoid maintenance, and passaging. We then detail procedures for TEC differentiation into cortical and medullary TEC lineages using defined growth factors, and techniques for analyzing organoids, including flow cytometry and qPCR. This protocol provides a valuable tool for studying TEC development and function. For complete details on the use and execution of this protocol, please refer to Lim et al.1
KW - Cell Biology
KW - Cell culture
KW - Molecular Biology
KW - Organoids
UR - http://www.scopus.com/inward/record.url?scp=105007624016&partnerID=8YFLogxK
U2 - 10.1016/j.xpro.2025.103883
DO - 10.1016/j.xpro.2025.103883
M3 - Article
AN - SCOPUS:105007624016
SN - 2666-1667
VL - 6
JO - STAR protocols
JF - STAR protocols
IS - 2
M1 - 103883
ER -