Progressive maturation toward hematopoietic stem cells in the mouse embryo aorta

Jean-Charles Boisset; Thomas Clapes; Anna Klaus; Natalie Papazian; Jos Onderwater; Mieke Mommaas-Kienhuis; Tom Cupedo; Catherine Robin

doi:10.1182/blood-2014-07-588954

Progressive maturation toward hematopoietic stem cells in the mouse embryo aorta

Jean-Charles Boisset, Thomas Clapes, Anna Klaus, Natalie Papazian, Jos Onderwater, Mieke Mommaas-Kienhuis, Tom Cupedo, Catherine Robin

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Clusters of cells attached to the endothelium of the main embryonic arteries were first observed a century ago. Present in most vertebrate species, such clusters, or intraaortic hematopoietic clusters (IAHCs), derive from specialized hemogenic endothelial cells and contain the first few hematopoietic stem cells (HSCs) generated during embryonic development. However, some discrepancies remained concerning the spatio-temporal appearance and the numbers of IAHCs and HSCs. Therefore, the exact cell composition and function of IAHCs remain unclear to date. We show here that IAHCs contain pre-HSCs (or HSC precursors) that can mature into HSCs in vivo (as shown by the successful long-term multilineage reconstitution of primary neonates and secondary adult recipients). Such IAHC pre-HSCs could contribute to the HSC pool increase observed at midgestation. The novel insights in pre-HSC to HSC transition represent an important step toward generating transplantable HSCs in vitro that are needed for autologous HSC transplantation therapies.

Original language	English
Pages (from-to)	465-9
Number of pages	5
Journal	Blood
Volume	125
Issue number	3
DOIs	https://doi.org/10.1182/blood-2014-07-588954
Publication status	Published - 15 Jan 2015

Keywords

Animals
Aorta
Cell Differentiation
Female
Hematopoietic Stem Cells
Mice
Organ Culture Techniques

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10.1182/blood-2014-07-588954

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title = "Progressive maturation toward hematopoietic stem cells in the mouse embryo aorta",

abstract = "Clusters of cells attached to the endothelium of the main embryonic arteries were first observed a century ago. Present in most vertebrate species, such clusters, or intraaortic hematopoietic clusters (IAHCs), derive from specialized hemogenic endothelial cells and contain the first few hematopoietic stem cells (HSCs) generated during embryonic development. However, some discrepancies remained concerning the spatio-temporal appearance and the numbers of IAHCs and HSCs. Therefore, the exact cell composition and function of IAHCs remain unclear to date. We show here that IAHCs contain pre-HSCs (or HSC precursors) that can mature into HSCs in vivo (as shown by the successful long-term multilineage reconstitution of primary neonates and secondary adult recipients). Such IAHC pre-HSCs could contribute to the HSC pool increase observed at midgestation. The novel insights in pre-HSC to HSC transition represent an important step toward generating transplantable HSCs in vitro that are needed for autologous HSC transplantation therapies.",

keywords = "Animals, Aorta, Cell Differentiation, Female, Hematopoietic Stem Cells, Mice, Organ Culture Techniques",

author = "Jean-Charles Boisset and Thomas Clapes and Anna Klaus and Natalie Papazian and Jos Onderwater and Mieke Mommaas-Kienhuis and Tom Cupedo and Catherine Robin",

note = "{\textcopyright} 2015 by The American Society of Hematology.",

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doi = "10.1182/blood-2014-07-588954",

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T1 - Progressive maturation toward hematopoietic stem cells in the mouse embryo aorta

AU - Boisset, Jean-Charles

AU - Clapes, Thomas

AU - Klaus, Anna

AU - Papazian, Natalie

AU - Onderwater, Jos

AU - Mommaas-Kienhuis, Mieke

AU - Cupedo, Tom

AU - Robin, Catherine

PY - 2015/1/15

Y1 - 2015/1/15

N2 - Clusters of cells attached to the endothelium of the main embryonic arteries were first observed a century ago. Present in most vertebrate species, such clusters, or intraaortic hematopoietic clusters (IAHCs), derive from specialized hemogenic endothelial cells and contain the first few hematopoietic stem cells (HSCs) generated during embryonic development. However, some discrepancies remained concerning the spatio-temporal appearance and the numbers of IAHCs and HSCs. Therefore, the exact cell composition and function of IAHCs remain unclear to date. We show here that IAHCs contain pre-HSCs (or HSC precursors) that can mature into HSCs in vivo (as shown by the successful long-term multilineage reconstitution of primary neonates and secondary adult recipients). Such IAHC pre-HSCs could contribute to the HSC pool increase observed at midgestation. The novel insights in pre-HSC to HSC transition represent an important step toward generating transplantable HSCs in vitro that are needed for autologous HSC transplantation therapies.

AB - Clusters of cells attached to the endothelium of the main embryonic arteries were first observed a century ago. Present in most vertebrate species, such clusters, or intraaortic hematopoietic clusters (IAHCs), derive from specialized hemogenic endothelial cells and contain the first few hematopoietic stem cells (HSCs) generated during embryonic development. However, some discrepancies remained concerning the spatio-temporal appearance and the numbers of IAHCs and HSCs. Therefore, the exact cell composition and function of IAHCs remain unclear to date. We show here that IAHCs contain pre-HSCs (or HSC precursors) that can mature into HSCs in vivo (as shown by the successful long-term multilineage reconstitution of primary neonates and secondary adult recipients). Such IAHC pre-HSCs could contribute to the HSC pool increase observed at midgestation. The novel insights in pre-HSC to HSC transition represent an important step toward generating transplantable HSCs in vitro that are needed for autologous HSC transplantation therapies.

KW - Animals

KW - Aorta

KW - Cell Differentiation

KW - Female

KW - Hematopoietic Stem Cells

KW - Mice

KW - Organ Culture Techniques

U2 - 10.1182/blood-2014-07-588954

DO - 10.1182/blood-2014-07-588954

M3 - Article

C2 - 25301706

SN - 0006-4971

VL - 125

SP - 465

EP - 469

JO - Blood

JF - Blood

IS - 3

ER -

Progressive maturation toward hematopoietic stem cells in the mouse embryo aorta

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Keywords

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