Prevalence of phylogroups and O25/ST131 in susceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands

I T M A Overdevest; A M C Bergmans; J J Verweij; J Vissers; N Bax; E Snelders; J A J W Kluytmans

doi:10.1016/j.cmi.2015.02.020

Prevalence of phylogroups and O25/ST131 in susceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands

I T M A Overdevest, A M C Bergmans, J J Verweij, J Vissers, N Bax, E Snelders, J A J W Kluytmans

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

To assess the distribution of phylogroups and O25/ST131 in the Netherlands, we performed a real-time polymerase chain reaction (PCR) on a collection of 108 wild-type Escherichia coli (WT-EC) and 134 extended-spectrum β-lactamase-producing E. coli (ESBL-EC). Phylogroup B2 was predominant, but ESBL-EC were less likely to belong to this phylogroup (48.5%) than were WT-EC (66.7%; p = 0.005). In WT-EC, phylogroups B2 and D seem to be more virulent, having a higher prevalence among midstream urine isolates and blood culture isolates, than in catheter-related urine isolates (83.3% and 87.9% vs. 61.9%; p 0.048). O25/ST131 is associated with ESBL production, being almost absent among phylogroup B2 WT-EC (61.5% vs. 5.6%; p < 0.001).

Original language	English
Pages (from-to)	570.e1–570.e4
Journal	Clinical Microbiology and Infection
Volume	21
Issue number	6
DOIs	https://doi.org/10.1016/j.cmi.2015.02.020
Publication status	Published - Jun 2015

Keywords

Adult
Aged
Aged, 80 and over
Escherichia coli
Escherichia coli Infections
Female
Genotype
Humans
Male
Middle Aged
Netherlands
Prevalence
Prospective Studies
Real-Time Polymerase Chain Reaction
Retrospective Studies
Serogroup
beta-Lactamases

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Overdevest, I. T. M. A., Bergmans, A. M. C., Verweij, J. J., Vissers, J., Bax, N., Snelders, E., & Kluytmans, J. A. J. W. (2015). Prevalence of phylogroups and O25/ST131 in susceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands. Clinical Microbiology and Infection, 21(6), 570.e1–570.e4. https://doi.org/10.1016/j.cmi.2015.02.020

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abstract = "To assess the distribution of phylogroups and O25/ST131 in the Netherlands, we performed a real-time polymerase chain reaction (PCR) on a collection of 108 wild-type Escherichia coli (WT-EC) and 134 extended-spectrum β-lactamase-producing E. coli (ESBL-EC). Phylogroup B2 was predominant, but ESBL-EC were less likely to belong to this phylogroup (48.5%) than were WT-EC (66.7%; p = 0.005). In WT-EC, phylogroups B2 and D seem to be more virulent, having a higher prevalence among midstream urine isolates and blood culture isolates, than in catheter-related urine isolates (83.3% and 87.9% vs. 61.9%; p 0.048). O25/ST131 is associated with ESBL production, being almost absent among phylogroup B2 WT-EC (61.5% vs. 5.6%; p < 0.001).",

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Prevalence of phylogroups and O25/ST131 in susceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands. / Overdevest, I T M A; Bergmans, A M C; Verweij, J J et al.
In: Clinical Microbiology and Infection, Vol. 21, No. 6, 06.2015, p. 570.e1–570.e4.

Research output: Contribution to journal › Article › Academic › peer-review

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AU - Bergmans, A M C

AU - Verweij, J J

AU - Vissers, J

AU - Bax, N

AU - Snelders, E

AU - Kluytmans, J A J W

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AB - To assess the distribution of phylogroups and O25/ST131 in the Netherlands, we performed a real-time polymerase chain reaction (PCR) on a collection of 108 wild-type Escherichia coli (WT-EC) and 134 extended-spectrum β-lactamase-producing E. coli (ESBL-EC). Phylogroup B2 was predominant, but ESBL-EC were less likely to belong to this phylogroup (48.5%) than were WT-EC (66.7%; p = 0.005). In WT-EC, phylogroups B2 and D seem to be more virulent, having a higher prevalence among midstream urine isolates and blood culture isolates, than in catheter-related urine isolates (83.3% and 87.9% vs. 61.9%; p 0.048). O25/ST131 is associated with ESBL production, being almost absent among phylogroup B2 WT-EC (61.5% vs. 5.6%; p < 0.001).

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KW - Escherichia coli Infections

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KW - Genotype

KW - Humans

KW - Male

KW - Middle Aged

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KW - Retrospective Studies

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JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

IS - 6

ER -

Prevalence of phylogroups and O25/ST131 in susceptible and extended-spectrum β-lactamase-producing Escherichia coli isolates, the Netherlands

Abstract

Keywords

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