Prediction of Muscle Energy States at Low Metabolic Rates Requires Feedback Control of Mitochondrial Respiratory Chain Activity by Inorganic Phosphate

Joep P. J. Schmitz; Jeroen A. L. Jeneson; Joep W. M. van Oorschot; Jeanine J. Prompers; Klaas Nicolay; Peter A. J. Hilbers; Natal A. W. van Riel

doi:10.1371/journal.pone.0034118

Prediction of Muscle Energy States at Low Metabolic Rates Requires Feedback Control of Mitochondrial Respiratory Chain Activity by Inorganic Phosphate

Joep P. J. Schmitz^*, Jeroen A. L. Jeneson, Joep W. M. van Oorschot, Jeanine J. Prompers, Klaas Nicolay, Peter A. J. Hilbers, Natal A. W. van Riel

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The regulation of the 100-fold dynamic range of mitochondrial ATP synthesis flux in skeletal muscle was investigated. Hypotheses of key control mechanisms were included in a biophysical model of oxidative phosphorylation and tested against metabolite dynamics recorded by 31 P nuclear magnetic resonance spectroscopy (P-31 MRS). Simulations of the initial model featuring only ADP and Pi feedback control of flux failed in reproducing the experimentally sampled relation between myoplasmic free energy of ATP hydrolysis (Delta G(p) = Delta Gp o'+RT In ([ADP][Pi]/[ATP]) and the rate of mitochondrial ATP synthesis at low fluxes (

Original language	English
Article number	34118
Number of pages	14
Journal	PLoS ONE [E]
Volume	7
Issue number	3
DOIs	https://doi.org/10.1371/journal.pone.0034118
Publication status	Published - 28 Mar 2012

Keywords

HUMAN SKELETAL-MUSCLE
OXIDATIVE-PHOSPHORYLATION
IN-VIVO
ATP HYDROLYSIS
SYSTEMS BIOLOGY
P-31 MRS
RECOVERY
ACTIVATION
ENERGETICS
EXERCISE

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10.1371/journal.pone.0034118

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Schmitz, J. P. J., Jeneson, J. A. L., van Oorschot, J. W. M., Prompers, J. J., Nicolay, K., Hilbers, P. A. J., & van Riel, N. A. W. (2012). Prediction of Muscle Energy States at Low Metabolic Rates Requires Feedback Control of Mitochondrial Respiratory Chain Activity by Inorganic Phosphate. PLoS ONE [E], 7(3), Article 34118. https://doi.org/10.1371/journal.pone.0034118

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title = "Prediction of Muscle Energy States at Low Metabolic Rates Requires Feedback Control of Mitochondrial Respiratory Chain Activity by Inorganic Phosphate",

abstract = "The regulation of the 100-fold dynamic range of mitochondrial ATP synthesis flux in skeletal muscle was investigated. Hypotheses of key control mechanisms were included in a biophysical model of oxidative phosphorylation and tested against metabolite dynamics recorded by 31 P nuclear magnetic resonance spectroscopy (P-31 MRS). Simulations of the initial model featuring only ADP and Pi feedback control of flux failed in reproducing the experimentally sampled relation between myoplasmic free energy of ATP hydrolysis (Delta G(p) = Delta Gp o'+RT In ([ADP][Pi]/[ATP]) and the rate of mitochondrial ATP synthesis at low fluxes (",

keywords = "HUMAN SKELETAL-MUSCLE, OXIDATIVE-PHOSPHORYLATION, IN-VIVO, ATP HYDROLYSIS, SYSTEMS BIOLOGY, P-31 MRS, RECOVERY, ACTIVATION, ENERGETICS, EXERCISE",

author = "Schmitz, {Joep P. J.} and Jeneson, {Jeroen A. L.} and {van Oorschot}, {Joep W. M.} and Prompers, {Jeanine J.} and Klaas Nicolay and Hilbers, {Peter A. J.} and {van Riel}, {Natal A. W.}",

year = "2012",

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doi = "10.1371/journal.pone.0034118",

language = "English",

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AU - Schmitz, Joep P. J.

AU - Jeneson, Jeroen A. L.

AU - van Oorschot, Joep W. M.

AU - Prompers, Jeanine J.

AU - Nicolay, Klaas

AU - Hilbers, Peter A. J.

AU - van Riel, Natal A. W.

PY - 2012/3/28

Y1 - 2012/3/28

N2 - The regulation of the 100-fold dynamic range of mitochondrial ATP synthesis flux in skeletal muscle was investigated. Hypotheses of key control mechanisms were included in a biophysical model of oxidative phosphorylation and tested against metabolite dynamics recorded by 31 P nuclear magnetic resonance spectroscopy (P-31 MRS). Simulations of the initial model featuring only ADP and Pi feedback control of flux failed in reproducing the experimentally sampled relation between myoplasmic free energy of ATP hydrolysis (Delta G(p) = Delta Gp o'+RT In ([ADP][Pi]/[ATP]) and the rate of mitochondrial ATP synthesis at low fluxes (

AB - The regulation of the 100-fold dynamic range of mitochondrial ATP synthesis flux in skeletal muscle was investigated. Hypotheses of key control mechanisms were included in a biophysical model of oxidative phosphorylation and tested against metabolite dynamics recorded by 31 P nuclear magnetic resonance spectroscopy (P-31 MRS). Simulations of the initial model featuring only ADP and Pi feedback control of flux failed in reproducing the experimentally sampled relation between myoplasmic free energy of ATP hydrolysis (Delta G(p) = Delta Gp o'+RT In ([ADP][Pi]/[ATP]) and the rate of mitochondrial ATP synthesis at low fluxes (

KW - HUMAN SKELETAL-MUSCLE

KW - OXIDATIVE-PHOSPHORYLATION

KW - IN-VIVO

KW - ATP HYDROLYSIS

KW - SYSTEMS BIOLOGY

KW - P-31 MRS

KW - RECOVERY

KW - ACTIVATION

KW - ENERGETICS

KW - EXERCISE

U2 - 10.1371/journal.pone.0034118

DO - 10.1371/journal.pone.0034118

M3 - Article

SN - 1932-6203

VL - 7

JO - PLoS ONE [E]

JF - PLoS ONE [E]

IS - 3

M1 - 34118

ER -

Prediction of Muscle Energy States at Low Metabolic Rates Requires Feedback Control of Mitochondrial Respiratory Chain Activity by Inorganic Phosphate

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Keywords

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