Platelet-activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca2+ in human neutrophils induced by opsonized particles

Anton T.J. Tool; Arthur J. Verhoeven; Dirk Roos; Leo Koenderman

doi:10.1016/0014-5793(89)81530-3

Platelet-activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca²⁺ in human neutrophils induced by opsonized particles

Anton T.J. Tool, Arthur J. Verhoeven^*, Dirk Roos, Leo Koenderman

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

22 Citations (Scopus)

Abstract

Additon of opsonized particles to human neutrophils in suspension leads to a biphasic elevation in the cytosolic free Ca²⁺ concentration ([Ca²⁺]_i). The rise in [Ca²⁺]_i, during the second phase (> 3 min) is pronounced (about 400 nM), in contrast to the rise during the first phase, which is relatively small (< 100 nM). The second and large rise in [Ca²⁺]_i is brought about by messenger(s) released from the cell after addition of opsonized particles. This second rise in [Ca²⁺]_i, is not observed in the presence of the platelet-activating factor (PAF) antagonist WEB 2086, indicating that PAF can act as an intercellular messenger affecting Ca²⁺ homeostasis in human neutrophils.

Original language	English
Pages (from-to)	209-212
Number of pages	4
Journal	FEBS letters
Volume	259
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(89)81530-3
Publication status	Published - 18 Dec 1989

Keywords

Human neutrophil
Intercellular messenger
Opsonized particle
Platelet-activating factor
[Ca], cytosolic free

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10.1016/0014-5793(89)81530-3

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title = "Platelet-activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca2+ in human neutrophils induced by opsonized particles",

abstract = "Additon of opsonized particles to human neutrophils in suspension leads to a biphasic elevation in the cytosolic free Ca2+ concentration ([Ca2+]i). The rise in [Ca2+]i, during the second phase (> 3 min) is pronounced (about 400 nM), in contrast to the rise during the first phase, which is relatively small (< 100 nM). The second and large rise in [Ca2+]i is brought about by messenger(s) released from the cell after addition of opsonized particles. This second rise in [Ca2+]i, is not observed in the presence of the platelet-activating factor (PAF) antagonist WEB 2086, indicating that PAF can act as an intercellular messenger affecting Ca2+ homeostasis in human neutrophils.",

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author = "Tool, \{Anton T.J.\} and Verhoeven, \{Arthur J.\} and Dirk Roos and Leo Koenderman",

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T1 - Platelet-activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca2+ in human neutrophils induced by opsonized particles

AU - Tool, Anton T.J.

AU - Verhoeven, Arthur J.

AU - Roos, Dirk

AU - Koenderman, Leo

PY - 1989/12/18

Y1 - 1989/12/18

N2 - Additon of opsonized particles to human neutrophils in suspension leads to a biphasic elevation in the cytosolic free Ca2+ concentration ([Ca2+]i). The rise in [Ca2+]i, during the second phase (> 3 min) is pronounced (about 400 nM), in contrast to the rise during the first phase, which is relatively small (< 100 nM). The second and large rise in [Ca2+]i is brought about by messenger(s) released from the cell after addition of opsonized particles. This second rise in [Ca2+]i, is not observed in the presence of the platelet-activating factor (PAF) antagonist WEB 2086, indicating that PAF can act as an intercellular messenger affecting Ca2+ homeostasis in human neutrophils.

AB - Additon of opsonized particles to human neutrophils in suspension leads to a biphasic elevation in the cytosolic free Ca2+ concentration ([Ca2+]i). The rise in [Ca2+]i, during the second phase (> 3 min) is pronounced (about 400 nM), in contrast to the rise during the first phase, which is relatively small (< 100 nM). The second and large rise in [Ca2+]i is brought about by messenger(s) released from the cell after addition of opsonized particles. This second rise in [Ca2+]i, is not observed in the presence of the platelet-activating factor (PAF) antagonist WEB 2086, indicating that PAF can act as an intercellular messenger affecting Ca2+ homeostasis in human neutrophils.

KW - Human neutrophil

KW - Intercellular messenger

KW - Opsonized particle

KW - Platelet-activating factor

KW - [Ca], cytosolic free

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DO - 10.1016/0014-5793(89)81530-3

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Platelet-activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca²⁺ in human neutrophils induced by opsonized particles

Abstract

Keywords

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