Platelet-activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca2+ in human neutrophils induced by opsonized particles

Anton T.J. Tool; Arthur J. Verhoeven; Dirk Roos; Leo Koenderman

doi:10.1016/0014-5793(89)81530-3

Platelet-activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca²⁺ in human neutrophils induced by opsonized particles

Anton T.J. Tool
, Arthur J. Verhoeven^*
, Dirk Roos
, Leo Koenderman

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

22 Citations (Scopus)

Abstract

Additon of opsonized particles to human neutrophils in suspension leads to a biphasic elevation in the cytosolic free Ca²⁺ concentration ([Ca²⁺]_i). The rise in [Ca²⁺]_i, during the second phase (> 3 min) is pronounced (about 400 nM), in contrast to the rise during the first phase, which is relatively small (< 100 nM). The second and large rise in [Ca²⁺]_i is brought about by messenger(s) released from the cell after addition of opsonized particles. This second rise in [Ca²⁺]_i, is not observed in the presence of the platelet-activating factor (PAF) antagonist WEB 2086, indicating that PAF can act as an intercellular messenger affecting Ca²⁺ homeostasis in human neutrophils.

Original language	English
Pages (from-to)	209-212
Number of pages	4
Journal	FEBS letters
Volume	259
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(89)81530-3
Publication status	Published - 18 Dec 1989

Keywords

Human neutrophil
Intercellular messenger
Opsonized particle
Platelet-activating factor
[Ca], cytosolic free

Access to Document

10.1016/0014-5793(89)81530-3

Cite this

@article{e76e7d2821814819a7647bfaf6d5cc3f,

title = "Platelet-activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca2+ in human neutrophils induced by opsonized particles",

abstract = "Additon of opsonized particles to human neutrophils in suspension leads to a biphasic elevation in the cytosolic free Ca2+ concentration ([Ca2+]i). The rise in [Ca2+]i, during the second phase (> 3 min) is pronounced (about 400 nM), in contrast to the rise during the first phase, which is relatively small (< 100 nM). The second and large rise in [Ca2+]i is brought about by messenger(s) released from the cell after addition of opsonized particles. This second rise in [Ca2+]i, is not observed in the presence of the platelet-activating factor (PAF) antagonist WEB 2086, indicating that PAF can act as an intercellular messenger affecting Ca2+ homeostasis in human neutrophils.",

keywords = "Human neutrophil, Intercellular messenger, Opsonized particle, Platelet-activating factor, [Ca], cytosolic free",

author = "Tool, \{Anton T.J.\} and Verhoeven, \{Arthur J.\} and Dirk Roos and Leo Koenderman",

year = "1989",

month = dec,

day = "18",

doi = "10.1016/0014-5793(89)81530-3",

language = "English",

volume = "259",

pages = "209--212",

journal = "FEBS letters",

issn = "0014-5793",

publisher = "John Wiley \& Sons Inc.",

number = "1",

}

TY - JOUR

T1 - Platelet-activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca2+ in human neutrophils induced by opsonized particles

AU - Tool, Anton T.J.

AU - Verhoeven, Arthur J.

AU - Roos, Dirk

AU - Koenderman, Leo

PY - 1989/12/18

Y1 - 1989/12/18

N2 - Additon of opsonized particles to human neutrophils in suspension leads to a biphasic elevation in the cytosolic free Ca2+ concentration ([Ca2+]i). The rise in [Ca2+]i, during the second phase (> 3 min) is pronounced (about 400 nM), in contrast to the rise during the first phase, which is relatively small (< 100 nM). The second and large rise in [Ca2+]i is brought about by messenger(s) released from the cell after addition of opsonized particles. This second rise in [Ca2+]i, is not observed in the presence of the platelet-activating factor (PAF) antagonist WEB 2086, indicating that PAF can act as an intercellular messenger affecting Ca2+ homeostasis in human neutrophils.

AB - Additon of opsonized particles to human neutrophils in suspension leads to a biphasic elevation in the cytosolic free Ca2+ concentration ([Ca2+]i). The rise in [Ca2+]i, during the second phase (> 3 min) is pronounced (about 400 nM), in contrast to the rise during the first phase, which is relatively small (< 100 nM). The second and large rise in [Ca2+]i is brought about by messenger(s) released from the cell after addition of opsonized particles. This second rise in [Ca2+]i, is not observed in the presence of the platelet-activating factor (PAF) antagonist WEB 2086, indicating that PAF can act as an intercellular messenger affecting Ca2+ homeostasis in human neutrophils.

KW - Human neutrophil

KW - Intercellular messenger

KW - Opsonized particle

KW - Platelet-activating factor

KW - [Ca], cytosolic free

UR - https://www.scopus.com/pages/publications/0024834968

U2 - 10.1016/0014-5793(89)81530-3

DO - 10.1016/0014-5793(89)81530-3

M3 - Article

C2 - 2599108

AN - SCOPUS:0024834968

SN - 0014-5793

VL - 259

SP - 209

EP - 212

JO - FEBS letters

JF - FEBS letters

IS - 1

ER -

Platelet-activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca²⁺ in human neutrophils induced by opsonized particles

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this