PID-1 is a novel factor that operates during 21U-RNA biogenesis in Caenorhabditis elegans

Bruno F M de Albuquerque; Maartje J Luteijn; Ricardo J Cordeiro Rodrigues; Petra van Bergeijk; Selma Waaijers; Lucas J T Kaaij; Holger Klein; Mike Boxem; René F Ketting

doi:10.1101/gad.238220.114

PID-1 is a novel factor that operates during 21U-RNA biogenesis in Caenorhabditis elegans

Bruno F M de Albuquerque, Maartje J Luteijn, Ricardo J Cordeiro Rodrigues, Petra van Bergeijk, Selma Waaijers, Lucas J T Kaaij, Holger Klein, Mike Boxem, René F Ketting

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The Piwi-piRNA pathway represents a small RNA-based mechanism responsible for the recognition and silencing of invading DNA. Biogenesis of piRNAs (21U-RNAs) is poorly understood. In Caenorhabditis elegans, the piRNA-binding Argonaute protein PRG-1 is the only known player acting downstream from precursor transcription. From a screen aimed at the isolation of piRNA-induced silencing-defective (Pid) mutations, we identified, among known Piwi pathway components, PID-1 as a novel player. PID-1 is a mostly cytoplasmic, germline-specific factor essential for 21U-RNA biogenesis, affecting an early step in the processing or transport of 21U precursor transcripts. We also show that maternal 21U-RNAs are essential to initiate silencing.

Original language	English
Pages (from-to)	683-688
Number of pages	6
Journal	Genes and Development
Volume	28
Issue number	7
DOIs	https://doi.org/10.1101/gad.238220.114
Publication status	Published - 1 Apr 2014

Keywords

Animals
Argonaute Proteins/genetics
Caenorhabditis elegans/genetics
Caenorhabditis elegans Proteins/genetics
Methylation
Mutation
Protein Precursors/metabolism
RNA Interference/physiology
RNA, Small Interfering/biosynthesis
Transgenes/genetics

Access to Document

10.1101/gad.238220.114

Cite this

@article{0768bdb952c04470ae4d5627fbd0cbf2,

title = "PID-1 is a novel factor that operates during 21U-RNA biogenesis in Caenorhabditis elegans",

abstract = "The Piwi-piRNA pathway represents a small RNA-based mechanism responsible for the recognition and silencing of invading DNA. Biogenesis of piRNAs (21U-RNAs) is poorly understood. In Caenorhabditis elegans, the piRNA-binding Argonaute protein PRG-1 is the only known player acting downstream from precursor transcription. From a screen aimed at the isolation of piRNA-induced silencing-defective (Pid) mutations, we identified, among known Piwi pathway components, PID-1 as a novel player. PID-1 is a mostly cytoplasmic, germline-specific factor essential for 21U-RNA biogenesis, affecting an early step in the processing or transport of 21U precursor transcripts. We also show that maternal 21U-RNAs are essential to initiate silencing.",

keywords = "Animals, Argonaute Proteins/genetics, Caenorhabditis elegans/genetics, Caenorhabditis elegans Proteins/genetics, Methylation, Mutation, Protein Precursors/metabolism, RNA Interference/physiology, RNA, Small Interfering/biosynthesis, Transgenes/genetics",

author = "{de Albuquerque}, {Bruno F M} and Luteijn, {Maartje J} and {Cordeiro Rodrigues}, {Ricardo J} and {van Bergeijk}, Petra and Selma Waaijers and Kaaij, {Lucas J T} and Holger Klein and Mike Boxem and Ketting, {Ren{\'e} F}",

note = "J PR 1 2014",

year = "2014",

month = apr,

day = "1",

doi = "10.1101/gad.238220.114",

language = "English",

volume = "28",

pages = "683--688",

journal = "Genes and Development",

issn = "0890-9369",

publisher = "Cold Spring Harbor Laboratory Press",

number = "7",

}

TY - JOUR

T1 - PID-1 is a novel factor that operates during 21U-RNA biogenesis in Caenorhabditis elegans

AU - de Albuquerque, Bruno F M

AU - Luteijn, Maartje J

AU - Cordeiro Rodrigues, Ricardo J

AU - van Bergeijk, Petra

AU - Waaijers, Selma

AU - Kaaij, Lucas J T

AU - Klein, Holger

AU - Boxem, Mike

AU - Ketting, René F

N1 - J PR 1 2014

PY - 2014/4/1

Y1 - 2014/4/1

N2 - The Piwi-piRNA pathway represents a small RNA-based mechanism responsible for the recognition and silencing of invading DNA. Biogenesis of piRNAs (21U-RNAs) is poorly understood. In Caenorhabditis elegans, the piRNA-binding Argonaute protein PRG-1 is the only known player acting downstream from precursor transcription. From a screen aimed at the isolation of piRNA-induced silencing-defective (Pid) mutations, we identified, among known Piwi pathway components, PID-1 as a novel player. PID-1 is a mostly cytoplasmic, germline-specific factor essential for 21U-RNA biogenesis, affecting an early step in the processing or transport of 21U precursor transcripts. We also show that maternal 21U-RNAs are essential to initiate silencing.

AB - The Piwi-piRNA pathway represents a small RNA-based mechanism responsible for the recognition and silencing of invading DNA. Biogenesis of piRNAs (21U-RNAs) is poorly understood. In Caenorhabditis elegans, the piRNA-binding Argonaute protein PRG-1 is the only known player acting downstream from precursor transcription. From a screen aimed at the isolation of piRNA-induced silencing-defective (Pid) mutations, we identified, among known Piwi pathway components, PID-1 as a novel player. PID-1 is a mostly cytoplasmic, germline-specific factor essential for 21U-RNA biogenesis, affecting an early step in the processing or transport of 21U precursor transcripts. We also show that maternal 21U-RNAs are essential to initiate silencing.

KW - Animals

KW - Argonaute Proteins/genetics

KW - Caenorhabditis elegans/genetics

KW - Caenorhabditis elegans Proteins/genetics

KW - Methylation

KW - Mutation

KW - Protein Precursors/metabolism

KW - RNA Interference/physiology

KW - RNA, Small Interfering/biosynthesis

KW - Transgenes/genetics

U2 - 10.1101/gad.238220.114

DO - 10.1101/gad.238220.114

M3 - Article

C2 - 24696453

SN - 0890-9369

VL - 28

SP - 683

EP - 688

JO - Genes and Development

JF - Genes and Development

IS - 7

ER -

PID-1 is a novel factor that operates during 21U-RNA biogenesis in Caenorhabditis elegans

Abstract

Keywords

Access to Document

Fingerprint

Cite this