Phenotypic and Molecular Characterization of Human Monoclonal TCRγ/δT‐Cell Lines from Jejunum and Colon of Healthy Individuals

R. J. ROBIJN; H. BLOEMENDAL; S. JAINANDUNSING; L. J.J.M. WIEGMAN; G. P. VANBERGE‐HENEGOUWEN; T. LOGTENBERG; J. C. KONINGSBERGER

doi:10.1111/j.1365-3083.1993.tb01721.x

Phenotypic and Molecular Characterization of Human Monoclonal TCR^γ/δT‐Cell Lines from Jejunum and Colon of Healthy Individuals

R. J. ROBIJN, H. BLOEMENDAL, S. JAINANDUNSING, L. J.J.M. WIEGMAN, G. P. VANBERGE‐HENEGOUWEN, T. LOGTENBERG, J. C. KONINGSBERGER^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We have perfortned a phenotypic and molecular analysis of monoclonal TCRγ/δ T‐cell lines derived from jejunal and colonic biopsies of healthy individuals. Flow cytometric analysis employing a panel of 24 monoclonal antibodies (MoAbs) demonstrated that intestinal TCRγ/δ intraepithelial lymphocytes (IEL) constitute a phenotypically heterogeneous population. Nucleotide sequence analysis of expressed TCRδ variable (V) regions revealed the dominant utilization of the Vδ2 and Dδ3 gene segments and frequent rearrangement of Jδ3. IEL Vδ regions displayed extensive junctional diversity as a result of N and P insertion and the utilization of Dδ3 in all three reading frames. The results demonstrate that intestinal TCRγ/δ T cells from healthy individuals constitute a phenotypically heterogeneous population expressing Vδ regions that differ from their systemic counterparts.

Original language	English
Pages (from-to)	247-253
Number of pages	7
Journal	Scandinavian Journal of Immunology
Volume	38
Issue number	3
DOIs	https://doi.org/10.1111/j.1365-3083.1993.tb01721.x
Publication status	Published - Sept 1993

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ROBIJN, R. J., BLOEMENDAL, H., JAINANDUNSING, S., WIEGMAN, L. J. J. M., VANBERGE‐HENEGOUWEN, G. P., LOGTENBERG, T., & KONINGSBERGER, J. C. (1993). Phenotypic and Molecular Characterization of Human Monoclonal TCR^γ/δT‐Cell Lines from Jejunum and Colon of Healthy Individuals. Scandinavian Journal of Immunology, 38(3), 247-253. https://doi.org/10.1111/j.1365-3083.1993.tb01721.x

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title = "Phenotypic and Molecular Characterization of Human Monoclonal TCRγ/δT‐Cell Lines from Jejunum and Colon of Healthy Individuals",

abstract = "We have perfortned a phenotypic and molecular analysis of monoclonal TCRγ/δ T‐cell lines derived from jejunal and colonic biopsies of healthy individuals. Flow cytometric analysis employing a panel of 24 monoclonal antibodies (MoAbs) demonstrated that intestinal TCRγ/δ intraepithelial lymphocytes (IEL) constitute a phenotypically heterogeneous population. Nucleotide sequence analysis of expressed TCRδ variable (V) regions revealed the dominant utilization of the Vδ2 and Dδ3 gene segments and frequent rearrangement of Jδ3. IEL Vδ regions displayed extensive junctional diversity as a result of N and P insertion and the utilization of Dδ3 in all three reading frames. The results demonstrate that intestinal TCRγ/δ T cells from healthy individuals constitute a phenotypically heterogeneous population expressing Vδ regions that differ from their systemic counterparts.",

author = "ROBIJN, {R. J.} and H. BLOEMENDAL and S. JAINANDUNSING and WIEGMAN, {L. J.J.M.} and VANBERGE‐HENEGOUWEN, {G. P.} and T. LOGTENBERG and KONINGSBERGER, {J. C.}",

year = "1993",

month = sep,

doi = "10.1111/j.1365-3083.1993.tb01721.x",

language = "English",

volume = "38",

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journal = "Scandinavian Journal of Immunology",

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ROBIJN, RJ, BLOEMENDAL, H, JAINANDUNSING, S, WIEGMAN, LJJM, VANBERGE‐HENEGOUWEN, GP, LOGTENBERG, T & KONINGSBERGER, JC 1993, 'Phenotypic and Molecular Characterization of Human Monoclonal TCR^γ/δT‐Cell Lines from Jejunum and Colon of Healthy Individuals', Scandinavian Journal of Immunology, vol. 38, no. 3, pp. 247-253. https://doi.org/10.1111/j.1365-3083.1993.tb01721.x

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T1 - Phenotypic and Molecular Characterization of Human Monoclonal TCRγ/δT‐Cell Lines from Jejunum and Colon of Healthy Individuals

AU - ROBIJN, R. J.

AU - BLOEMENDAL, H.

AU - JAINANDUNSING, S.

AU - WIEGMAN, L. J.J.M.

AU - VANBERGE‐HENEGOUWEN, G. P.

AU - LOGTENBERG, T.

AU - KONINGSBERGER, J. C.

PY - 1993/9

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N2 - We have perfortned a phenotypic and molecular analysis of monoclonal TCRγ/δ T‐cell lines derived from jejunal and colonic biopsies of healthy individuals. Flow cytometric analysis employing a panel of 24 monoclonal antibodies (MoAbs) demonstrated that intestinal TCRγ/δ intraepithelial lymphocytes (IEL) constitute a phenotypically heterogeneous population. Nucleotide sequence analysis of expressed TCRδ variable (V) regions revealed the dominant utilization of the Vδ2 and Dδ3 gene segments and frequent rearrangement of Jδ3. IEL Vδ regions displayed extensive junctional diversity as a result of N and P insertion and the utilization of Dδ3 in all three reading frames. The results demonstrate that intestinal TCRγ/δ T cells from healthy individuals constitute a phenotypically heterogeneous population expressing Vδ regions that differ from their systemic counterparts.

AB - We have perfortned a phenotypic and molecular analysis of monoclonal TCRγ/δ T‐cell lines derived from jejunal and colonic biopsies of healthy individuals. Flow cytometric analysis employing a panel of 24 monoclonal antibodies (MoAbs) demonstrated that intestinal TCRγ/δ intraepithelial lymphocytes (IEL) constitute a phenotypically heterogeneous population. Nucleotide sequence analysis of expressed TCRδ variable (V) regions revealed the dominant utilization of the Vδ2 and Dδ3 gene segments and frequent rearrangement of Jδ3. IEL Vδ regions displayed extensive junctional diversity as a result of N and P insertion and the utilization of Dδ3 in all three reading frames. The results demonstrate that intestinal TCRγ/δ T cells from healthy individuals constitute a phenotypically heterogeneous population expressing Vδ regions that differ from their systemic counterparts.

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Phenotypic and Molecular Characterization of Human Monoclonal TCR^γ/δT‐Cell Lines from Jejunum and Colon of Healthy Individuals

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