Particulate 3D Hydrogels of Silk Fibroin-Pluronic to Deliver Curcumin for Infection-Free Wound Healing

Azin Khodaei*, Narges Johari, Fatemeh Jahanmard, Leonardo Cecotto, Sadjad Khosravimelal, Hamid Reza Madaah Hosseini, Reza Bagheri*, Ali Samadikuchaksaraei, Saber Amin Yavari*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Skin is the largest protective tissue of the body and is at risk of damage. Hence, the design and development of wound dressing materials is key for tissue repair and regeneration. Although silk fibroin is a known biopolymer in tissue engineering, its degradation rate is not correlated with wound closure rate. To address this disadvantage, we mimicked the hierarchical structure of skin and also provided antibacterial properties; a hydrogel with globular structure consisting of silk fibroin, pluronic F127, and curcumin was developed. In this regard, the effect of pluronic and curcumin on the structural and mechanical properties of the hydrogel was studied. The results showed that curcumin affected the particle size, crystallinity, and ultimate elongation of the hydrogels. In vitro assays confirmed that the hydrogel containing curcumin is not cytotoxic while the diffused curcumin and pluronic provided a considerable bactericidal property against Methicillin-resistant Staphylococcus aureus. Interestingly, presence of pluronic caused more than a 99% reduction in planktonic and adherent bacteria in the curcumin-free hydrogel groups. Moreover, curcumin improved this number further and inhibited bacteria adhesion to prevent biofilm formation. Overall, the developed hydrogel showed the potential to be used for skin tissue regeneration.

Original languageEnglish
Article number483
JournalBiomimetics
Volume9
Issue number8
DOIs
Publication statusPublished - Aug 2024

Keywords

  • hierarchical
  • MRSA
  • Poloxamer 407
  • scaffolds
  • wound healing

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