Parathyroid hormone-like effects of rainbow trout stannius products on bone resorption of embryonic mouse calvaria in vitro

Floris P.J.G. Lafeber; H. I.M.B. Schaefer; M. P.M. Herrmann-erlee; S. E.Wendelaar Bonga

doi:10.1210/endo-119-5-2249

Parathyroid hormone-like effects of rainbow trout stannius products on bone resorption of embryonic mouse calvaria in vitro

Floris P.J.G. Lafeber^*
, H. I.M.B. Schaefer
, M. P.M. Herrmann-erlee
, S. E.Wendelaar Bonga

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

19 Citations (Scopus)

Abstract

Products of the Stannius corpuscles (SC) of rainbow trout were tested in an established PTH bioassay involving bone resorption in embryonic mouse calvaria. Aqueous extracts from Stannius corpuscles (SC-homogenate) showed a bone-resorbing activity comparable to PTH in 24-h cultures of calvaria, indicated by a dose-dependent stimulation of lactate production and of calcium, phosphate as well as β-glucuronidase release. Moreover, SC-homogenates induced an increase in osteoclastic activity. The PTH-like SC-principle is released during in vitro incubations of the glands. These results and the lack of an additive effect of SC-products and PTH on bone resorption suggest that both products activate the same receptor. We hypothesize that the hypocalcemic hormone of the SC of fish shares structural resemblance with PTH, the major hypercalcemic hormone of terrestrial vertebrates.

Original language	English
Pages (from-to)	2249-2255
Number of pages	7
Journal	Endocrinology
Volume	119
Issue number	5
DOIs	https://doi.org/10.1210/endo-119-5-2249
Publication status	Published - Nov 1986
Externally published	Yes

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title = "Parathyroid hormone-like effects of rainbow trout stannius products on bone resorption of embryonic mouse calvaria in vitro",

abstract = "Products of the Stannius corpuscles (SC) of rainbow trout were tested in an established PTH bioassay involving bone resorption in embryonic mouse calvaria. Aqueous extracts from Stannius corpuscles (SC-homogenate) showed a bone-resorbing activity comparable to PTH in 24-h cultures of calvaria, indicated by a dose-dependent stimulation of lactate production and of calcium, phosphate as well as β-glucuronidase release. Moreover, SC-homogenates induced an increase in osteoclastic activity. The PTH-like SC-principle is released during in vitro incubations of the glands. These results and the lack of an additive effect of SC-products and PTH on bone resorption suggest that both products activate the same receptor. We hypothesize that the hypocalcemic hormone of the SC of fish shares structural resemblance with PTH, the major hypercalcemic hormone of terrestrial vertebrates.",

author = "Lafeber, \{Floris P.J.G.\} and Schaefer, \{H. I.M.B.\} and Herrmann-erlee, \{M. P.M.\} and Bonga, \{S. E.Wendelaar\}",

year = "1986",

month = nov,

doi = "10.1210/endo-119-5-2249",

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T1 - Parathyroid hormone-like effects of rainbow trout stannius products on bone resorption of embryonic mouse calvaria in vitro

AU - Lafeber, Floris P.J.G.

AU - Schaefer, H. I.M.B.

AU - Herrmann-erlee, M. P.M.

AU - Bonga, S. E.Wendelaar

PY - 1986/11

Y1 - 1986/11

N2 - Products of the Stannius corpuscles (SC) of rainbow trout were tested in an established PTH bioassay involving bone resorption in embryonic mouse calvaria. Aqueous extracts from Stannius corpuscles (SC-homogenate) showed a bone-resorbing activity comparable to PTH in 24-h cultures of calvaria, indicated by a dose-dependent stimulation of lactate production and of calcium, phosphate as well as β-glucuronidase release. Moreover, SC-homogenates induced an increase in osteoclastic activity. The PTH-like SC-principle is released during in vitro incubations of the glands. These results and the lack of an additive effect of SC-products and PTH on bone resorption suggest that both products activate the same receptor. We hypothesize that the hypocalcemic hormone of the SC of fish shares structural resemblance with PTH, the major hypercalcemic hormone of terrestrial vertebrates.

AB - Products of the Stannius corpuscles (SC) of rainbow trout were tested in an established PTH bioassay involving bone resorption in embryonic mouse calvaria. Aqueous extracts from Stannius corpuscles (SC-homogenate) showed a bone-resorbing activity comparable to PTH in 24-h cultures of calvaria, indicated by a dose-dependent stimulation of lactate production and of calcium, phosphate as well as β-glucuronidase release. Moreover, SC-homogenates induced an increase in osteoclastic activity. The PTH-like SC-principle is released during in vitro incubations of the glands. These results and the lack of an additive effect of SC-products and PTH on bone resorption suggest that both products activate the same receptor. We hypothesize that the hypocalcemic hormone of the SC of fish shares structural resemblance with PTH, the major hypercalcemic hormone of terrestrial vertebrates.

UR - https://www.scopus.com/pages/publications/0022994796

U2 - 10.1210/endo-119-5-2249

DO - 10.1210/endo-119-5-2249

M3 - Article

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AN - SCOPUS:0022994796

SN - 0013-7227

VL - 119

SP - 2249

EP - 2255

JO - Endocrinology

JF - Endocrinology

IS - 5

ER -

Parathyroid hormone-like effects of rainbow trout stannius products on bone resorption of embryonic mouse calvaria in vitro

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