TY - JOUR
T1 - Parathyroid hormone-like effects of rainbow trout stannius products on bone resorption of embryonic mouse calvaria in vitro
AU - Lafeber, Floris P.J.G.
AU - Schaefer, H. I.M.B.
AU - Herrmann-erlee, M. P.M.
AU - Bonga, S. E.Wendelaar
PY - 1986/11
Y1 - 1986/11
N2 - Products of the Stannius corpuscles (SC) of rainbow trout were tested in an established PTH bioassay involving bone resorption in embryonic mouse calvaria. Aqueous extracts from Stannius corpuscles (SC-homogenate) showed a bone-resorbing activity comparable to PTH in 24-h cultures of calvaria, indicated by a dose-dependent stimulation of lactate production and of calcium, phosphate as well as β-glucuronidase release. Moreover, SC-homogenates induced an increase in osteoclastic activity. The PTH-like SC-principle is released during in vitro incubations of the glands. These results and the lack of an additive effect of SC-products and PTH on bone resorption suggest that both products activate the same receptor. We hypothesize that the hypocalcemic hormone of the SC of fish shares structural resemblance with PTH, the major hypercalcemic hormone of terrestrial vertebrates.
AB - Products of the Stannius corpuscles (SC) of rainbow trout were tested in an established PTH bioassay involving bone resorption in embryonic mouse calvaria. Aqueous extracts from Stannius corpuscles (SC-homogenate) showed a bone-resorbing activity comparable to PTH in 24-h cultures of calvaria, indicated by a dose-dependent stimulation of lactate production and of calcium, phosphate as well as β-glucuronidase release. Moreover, SC-homogenates induced an increase in osteoclastic activity. The PTH-like SC-principle is released during in vitro incubations of the glands. These results and the lack of an additive effect of SC-products and PTH on bone resorption suggest that both products activate the same receptor. We hypothesize that the hypocalcemic hormone of the SC of fish shares structural resemblance with PTH, the major hypercalcemic hormone of terrestrial vertebrates.
UR - http://www.scopus.com/inward/record.url?scp=0022994796&partnerID=8YFLogxK
U2 - 10.1210/endo-119-5-2249
DO - 10.1210/endo-119-5-2249
M3 - Article
C2 - 3769870
AN - SCOPUS:0022994796
SN - 0013-7227
VL - 119
SP - 2249
EP - 2255
JO - Endocrinology
JF - Endocrinology
IS - 5
ER -