@article{420f6ef696b14991ab2d03ef44b61d90,
title = "Organoids and organs-on-chips: Insights into human gut-microbe interactions",
abstract = "The important and diverse roles of the gut microbiota in human health and disease are increasingly recognized. The difficulty of inferring causation from metagenomic microbiome sequencing studies and from mouse-human interspecies differences has prompted the development of sophisticated in vitro models of human gut-microbe interactions. Here, we review recent advances in the co-culture of microbes with intestinal and colonic epithelia, comparing the rapidly developing fields of organoids and organs-on-chips with other standard models. We describe how specific individual processes by which microbes and epithelia interact can be recapitulated in vitro. Using examples of bacterial, viral, and parasitic infections, we highlight the advantages of each culture model and discuss current trends and future possibilities to build more complex co-cultures.",
keywords = "bacteria, co-culture, epithelium, host, in vitro models, infection, intestine, microbe, microbiota, organ-on-a-chip, organoid, virus",
author = "Jens Puschhof and Cayetano Pleguezuelos-Manzano and Hans Clevers",
note = "Funding Information: This work was supported by a CRUK grant OPTIMISTIC ( C10674/A27140 ), the Netherlands Organ-on-Chip Initiative ( 024.003.001 ) from the Netherlands Organization for Scientific Research ( NWO ), Culture and Science of the Government of the Netherlands, the Oncode Institute (partly financed by the Dutch Cancer Society) the European Research Council under ERC Advanced grant agreement no. 67013. Funding Information: This work was supported by a CRUK grant OPTIMISTIC (C10674/A27140), the Netherlands Organ-on-Chip Initiative (024.003.001) from the Netherlands Organization for Scientific Research (NWO), Culture and Science of the Government of the Netherlands, the Oncode Institute (partly financed by the Dutch Cancer Society) the European Research Council under ERC Advanced grant agreement no. 67013. H.C. is an inventor on multiple patents held by the Dutch Royal Netherlands Academy of Arts and Sciences that cover organoid technology. H.C. is a member of the board of directors of Roche. H.C.?s full disclosure is given at https://www.uu.nl/staff/JCClevers/. J.P. and C.P.-M. declare no competing interests. Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = jun,
day = "9",
doi = "10.1016/j.chom.2021.04.002",
language = "English",
volume = "29",
pages = "867--878",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "6",
}