Organoid technology as an emerging tool for gynecological oncology research: Dawn of precision medicine in cancer research

Kadi Lõhmussaar

Research output: ThesisDoctoral thesis 2 (Research NOT UU / Graduation UU)

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Abstract

Organoid technology is an emerging new model system that enables to closely capture
and study the physiology and properties of the epithelial tissues “in a dish”. The model
relies on the presence of stem cell pools within the tissue of interest and their intrinsic
patterning capacity. Under defined niche factors and support from extracellular membrane-like scaffolds, these cells are able to self-organize into three-dimensional structures that closely resemble the architecture and properties of source tissue, creating unparalleled
opportunities. Since the invention in intestine, organoid technology has been applied to
many additional epithelial tissues, helping to gain valuable insight into their biology both
in homeostatic and diseased conditions. More recently, the model has also been adapted
to study the female reproductive tract (FRT) and associated disorders. In this thesis we
describe the establishment and characterization of a broad panel of 3D organoid cultures
from a variety of epithelial gynecological cancers and respective healthy tissues of origin
with the purpose to better understand the heterogeneity and the individual characteristics
of these tumors.
Original languageEnglish
Awarding Institution
  • University Medical Center (UMC) Utrecht
Supervisors/Advisors
  • Clevers, Hans, Primary supervisor
Award date17 Nov 2020
Publisher
Print ISBNs978-94-93197-33-6
DOIs
Publication statusPublished - 17 Nov 2020
Externally publishedYes

Keywords

  • Gynecological oncology
  • organoids
  • CRISPR-Cas9

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