Oral human papillomavirus infection in HIV-negative and HIV-infected MSM

S.H. Mooij, H.J. Boot, A.G.C.L. Speksnijder, I.G. Stolte, C.J.L.M. Meijer, P.J.F. Snijders, D.W.M. Verhagen, A.J. King, H.J. de Vries, W. Quint, M.A.B. van der Sande, M.F. Loeff

Research output: Contribution to journalArticleAcademicpeer-review


OBJECTIVE: Oral infection with human papillomavirus (HPV) is associated with a subset of head and neck cancers. We compared prevalence of, and risk factors for, oral HPV infection among HIV-negative and HIV-infected MSM.

DESIGN: Analysis of baseline data from a prospective cohort study.

METHODS: MSM aged 18 years or older were recruited from three study sites in Amsterdam, the Netherlands. Participants completed a self-administered risk-factor questionnaire. Oral-rinse and gargle specimens were analyzed for HPV DNA and genotyped using a highly sensitive PCR and reverse line blot assay [short PCR fragment (SPF)10-PCR-DNA Enzyme Immuno Assay (DEIA)/LiPA25 system].

RESULTS: In 2010-2011, 794 MSM were included, of whom 767 participants had sufficient data for analysis. Median age was 40.1 years [interquartile range (IQR) 34.8-47.5] and 314 men were HIV-infected (40.9%). Any of 25 typable HPV types was present in 24.4% of all oral samples. Oncogenic HPV types were detected in 24.8 and 8.8% of oral samples from HIV-infected and HIV-negative MSM, respectively (P < 0.001). Of these high-risk types, HPV-16 was the most common (overall 3.4%). Oral infection with high-risk HPV was associated with HIV infection in multivariable analysis (P < 0.001). Increasing age was significantly associated with oral HPV infection in HIV-negative, but not in HIV-infected MSM.

CONCLUSION: Oral HPV infection is very common among MSM. HIV infection was independently associated with high-risk oral HPV infection, suggesting an important role of HIV in oral HPV infection.

Original languageEnglish
Pages (from-to)2117-2128
Number of pages12
Issue number13
Publication statusPublished - 24 Aug 2013


  • Adult
  • Cohort Studies
  • DNA, Viral
  • HIV Infections
  • Homosexuality, Male
  • Humans
  • Immunoenzyme Techniques
  • Male
  • Middle Aged
  • Mouth
  • Mouth Diseases
  • Netherlands
  • Papillomaviridae
  • Papillomavirus Infections
  • Polymerase Chain Reaction
  • Prevalence
  • Questionnaires
  • Retrospective Studies
  • Risk Factors
  • Young Adult


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