Oligonucleotides

Raymond M. Schiffelers; Enrico Mastrobattista

doi:10.1007/978-1-4614-6486-0_23

Oligonucleotides

Raymond M. Schiffelers^*, Enrico Mastrobattista

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

Oligonucleotides are (short) chains of (chemically modified) ribo- or deoxyribonucleotides. Their ability to bind to chromosomal DNA, mRNA, or non-coding RNA (ncRNA) through Watson-Crick and Hoogsteen base pairing offers possibilities for highly specific intervention in gene transcription, mRNA translation, gene repair, and recombination for therapeutic applications. In theory, a sequence of 15–17 bases occurs only once in the human genome, which would allow specific manipulation of single genes for oligonucleotides in this size range. In addition, therapeutic effects of oligonucleotides can be obtained through sequence-specific binding of transcription factors and intramolecular folding into structures that can bind to and interfere with the function of various biomolecules. Finally, cells display specific receptors for oligonucleotides. These receptors can activate a variety of immunological responses that can be of therapeutic value.

Original language	English
Title of host publication	Pharmaceutical Biotechnology
Subtitle of host publication	Fundamentals and Applications, Fourth Edition
Publisher	Springer New York
Pages	459-475
Number of pages	17
ISBN (Electronic)	9781461464860
ISBN (Print)	9781461464853
DOIs	https://doi.org/10.1007/978-1-4614-6486-0_23
Publication status	Published - 1 Jan 2013

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title = "Oligonucleotides",

abstract = "Oligonucleotides are (short) chains of (chemically modified) ribo- or deoxyribonucleotides. Their ability to bind to chromosomal DNA, mRNA, or non-coding RNA (ncRNA) through Watson-Crick and Hoogsteen base pairing offers possibilities for highly specific intervention in gene transcription, mRNA translation, gene repair, and recombination for therapeutic applications. In theory, a sequence of 15–17 bases occurs only once in the human genome, which would allow specific manipulation of single genes for oligonucleotides in this size range. In addition, therapeutic effects of oligonucleotides can be obtained through sequence-specific binding of transcription factors and intramolecular folding into structures that can bind to and interfere with the function of various biomolecules. Finally, cells display specific receptors for oligonucleotides. These receptors can activate a variety of immunological responses that can be of therapeutic value.",

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AB - Oligonucleotides are (short) chains of (chemically modified) ribo- or deoxyribonucleotides. Their ability to bind to chromosomal DNA, mRNA, or non-coding RNA (ncRNA) through Watson-Crick and Hoogsteen base pairing offers possibilities for highly specific intervention in gene transcription, mRNA translation, gene repair, and recombination for therapeutic applications. In theory, a sequence of 15–17 bases occurs only once in the human genome, which would allow specific manipulation of single genes for oligonucleotides in this size range. In addition, therapeutic effects of oligonucleotides can be obtained through sequence-specific binding of transcription factors and intramolecular folding into structures that can bind to and interfere with the function of various biomolecules. Finally, cells display specific receptors for oligonucleotides. These receptors can activate a variety of immunological responses that can be of therapeutic value.

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Oligonucleotides

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