Abstract
Human immunodeficiency virus type 1 (HIV-1) is classified into different phylogenetic subtypes, with subtype C representing more than half of the novel infections globally. However, there are relatively few subtype C envelopes available for study. We amplified 18 unique env genes from 13 patients who were infected with subtype C HIV-1 in six African countries and in Scotland to create replication-competent viruses. These envelopes are phylogenetically diverse across the subtype C spectrum, and have on average more N-linked glycosylation sites and slightly longer variable loops than previously described C envelopes. We found that CCR3 coreceptor usage is less prevalent in subtype C than in subtype B viruses, and these envelopes have varied sensitivity to neutralization. The subtype C chimeric viruses generated in this study will be useful for evaluating the breadth of neutralizing antibodies and other entry inhibitors.
Original language | English |
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Pages (from-to) | 2374-80 |
Number of pages | 7 |
Journal | Journal of General Virology |
Volume | 91 |
Issue number | Pt 9 |
DOIs | |
Publication status | Published - Sept 2010 |
Externally published | Yes |
Keywords
- Africa
- Cloning, Molecular
- Genes, env
- Glycosylation
- HIV Antigens/chemistry
- HIV Infections/blood
- HIV-1/classification
- Host-Pathogen Interactions/genetics
- Humans
- Neutralization Tests
- Phylogeny
- Receptors, CCR3/physiology
- Receptors, HIV/physiology
- Scotland
- env Gene Products, Human Immunodeficiency Virus/chemistry