Novel subtype C human immunodeficiency virus type 1 envelopes cloned directly from plasma: coreceptor usage and neutralization phenotypes

Willie W L Koh, Anna Forsman, Stéphane Hué, Gisela J van der Velden, David L Yirrell, Aine McKnight, Robin A Weiss, Marlén M I Aasa-Chapman

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Human immunodeficiency virus type 1 (HIV-1) is classified into different phylogenetic subtypes, with subtype C representing more than half of the novel infections globally. However, there are relatively few subtype C envelopes available for study. We amplified 18 unique env genes from 13 patients who were infected with subtype C HIV-1 in six African countries and in Scotland to create replication-competent viruses. These envelopes are phylogenetically diverse across the subtype C spectrum, and have on average more N-linked glycosylation sites and slightly longer variable loops than previously described C envelopes. We found that CCR3 coreceptor usage is less prevalent in subtype C than in subtype B viruses, and these envelopes have varied sensitivity to neutralization. The subtype C chimeric viruses generated in this study will be useful for evaluating the breadth of neutralizing antibodies and other entry inhibitors.

Original languageEnglish
Pages (from-to)2374-80
Number of pages7
JournalJournal of General Virology
Volume91
Issue numberPt 9
DOIs
Publication statusPublished - Sept 2010
Externally publishedYes

Keywords

  • Africa
  • Cloning, Molecular
  • Genes, env
  • Glycosylation
  • HIV Antigens/chemistry
  • HIV Infections/blood
  • HIV-1/classification
  • Host-Pathogen Interactions/genetics
  • Humans
  • Neutralization Tests
  • Phylogeny
  • Receptors, CCR3/physiology
  • Receptors, HIV/physiology
  • Scotland
  • env Gene Products, Human Immunodeficiency Virus/chemistry

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