Abstract
CC chemokine ligand 18/dendritic cell-chemokine 1 (CCL18/DC-CK1) is a CC chemokine, preferentially expressed by DC, which acts as a chemoattractant for naive T cells and mantle zone B cells. Applying a newly developed CCL18/DC-CK1 sandwich enzyme-linked immunosorbent assay, we demonstrate that DC secrete high amounts of CCL18/DC-CK1 and that this expression can be increased by interleukin-10. High levels of CCL18/DC-CK1 were also detected in human serum (average of 88 ng/ml). Moreover, elevated CCL18/DC-CK1 levels were detected in synovial fluid from rheumatoid arthritis patients and in drain fluid (average of 254 ng/ml and 122 ng/ml, respectively). Immunoprecipitation experiment using anti-CCL18/DC-CK1 monoclonal antibodies revealed a protein of 6-7 kDa in serum and drain fluid that was indistinguishable from recombinant CCL18/DC-CK1 on Western blot and in re-aggregation assays. The concentration of CCL18/DC-CK1 found in human serum is in the same order of magnitude as was previously reported to completely inhibit CCL11/eotaxin-induced CC chemokine receptor 3 (CCR3) activation and consequent migration of eosinophils. CCL18/DC-CK1 may therefore function as an agonist (for naive T and B cells) and as an antagonist for CCR3-expressing leukocytes such as eosinophils.
Original language | English |
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Pages (from-to) | 739-47 |
Number of pages | 9 |
Journal | Journal of Leukocyte Biology |
Volume | 77 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2005 |
Externally published | Yes |
Keywords
- Antibodies, Monoclonal
- Antibody Specificity
- Arthritis, Rheumatoid
- Body Fluids
- Cells, Cultured
- Chemokines, CC
- Chemotaxis, Leukocyte
- Dendritic Cells
- Enzyme-Linked Immunosorbent Assay
- Humans
- Interleukin-10
- Leukocytes
- Receptors, CCR3
- Receptors, Chemokine
- Recombinant Proteins
- Journal Article
- Research Support, Non-U.S. Gov't