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Novel genotypes, phenotypes, and triggers in humans with OTULIN haploinsufficiency

  • Tristan J van der Linden
  • , Rob J W Arts
  • , Catherine M Biggs
  • , Laleh Habibi
  • , Laura Batlle-Masó
  • , Arjan van Laarhoven
  • , Lisette M Scheepmaker
  • , Pariya Yousefi
  • , Andrea C Gómez-Raccio
  • , Zahra Alizadeh
  • , Catharina M Mulders-Manders
  • , Jaap Ten Oever
  • , Janneke Schuurs-Hoeijmakers
  • , Nasrin Alipour-Olyei
  • , Anne Molitor
  • , Daniela Di Giovanni
  • , Raphael Carapito
  • , Seiamak Bahram
  • , Gisela Seminario
  • , Liliana Bezrodnik
  • Mana Momenilandi, Mohammad Shahrooei, Jacinta Bustamante, Ivona Aksentijevich, Daniel Kastner, Mohammad R Fazlollahi, Roger Colobran, Stuart E Turvey, Frank L van de Veerdonk, Jean-Laurent Casanova, Bertrand Boisson, Bart W Bardoel, András N Spaan*
*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Human OTULIN haploinsufficiency predisposes to life-threatening necrosis of the skin and lungs. Disease is triggered by infectious agents, typically Staphylococcus aureus, as well as unknown etiologies. We describe and characterize six unrelated patients who carry rare, predicted deleterious variants of OTULIN in heterozygosity. In addition to staphylococcal infections, the disease in the patients is elicited by previously underappreciated triggers, including mechanical or iatrogenic traumas and pseudomonal or clostridial infections. Severe necrosis of the lungs and/or skin are clinical hallmarks of their disease. By combining in vitro allele characterizations and functional studies in patients' cells, we demonstrate that the patients suffer from OTULIN haploinsufficiency. We provide guidance for assessing heterozygous OTULIN variants in diagnostic settings by evaluating in silico measures of predicted deleteriousness. The clinical course of the patients expands the genotypic and phenotypic spectrum of OTULIN haploinsufficiency and provides, in the light of a broadening of triggers, leads for therapeutic interventions.

Original languageEnglish
Article numbere20250018
Number of pages20
JournalJournal of human immunity
Volume1
Issue number4
DOIs
Publication statusPublished - 3 Nov 2025

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