No association between IL-10 promoter gene polymorphism and heart failure or rejection following cardiac transplantation

F.J. Bijlsma; A.H. Bruggink; M. Hartman; F.H.J. Gmelig Meyling; M.G.J. Tilanus; N. de Jonge; R.A. de Weger

No association between IL-10 promoter gene polymorphism and heart failure or rejection following cardiac transplantation

F.J. Bijlsma, A.H. Bruggink, M. Hartman, F.H.J. Gmelig Meyling, M.G.J. Tilanus, N. de Jonge, R.A. de Weger

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Expression of interleukin (IL)-10 influences the frequency of rejection events after organ transplantation. Therefore, 70 heart transplant patients were genotyped for three single nucleotide polymorphisms and a microsatellite polymorphism in the promotor region of the IL-10 gene. The promoter region was amplified by polymerase chain reaction and genotyped by a colorometric oligo ligation assay and gene scan analysis, respectively. Patient groups consisted of patients suffering from dilated cardiomyopathy or ischaemic heart disease. Cardiac donors served as control group. No correlation was found between genotypes and heart failure or rejection after heart transplantation. This may indicate that in heart transplantation, the total balance of cytokine production is more important for post-transplant rejection activities than the levels of IL-10 as such.

Original language	English
Pages (from-to)	151-153
Number of pages	3
Journal	Tissue Antigens
Volume	57
Issue number	2
Publication status	Published - Feb 2001

Keywords

Gene Expression
Graft Rejection
Heart Failure
Heart Transplantation
Humans
Interleukin-10
Polymorphism, Single Nucleotide
Promoter Regions, Genetic

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title = "No association between IL-10 promoter gene polymorphism and heart failure or rejection following cardiac transplantation",

abstract = "Expression of interleukin (IL)-10 influences the frequency of rejection events after organ transplantation. Therefore, 70 heart transplant patients were genotyped for three single nucleotide polymorphisms and a microsatellite polymorphism in the promotor region of the IL-10 gene. The promoter region was amplified by polymerase chain reaction and genotyped by a colorometric oligo ligation assay and gene scan analysis, respectively. Patient groups consisted of patients suffering from dilated cardiomyopathy or ischaemic heart disease. Cardiac donors served as control group. No correlation was found between genotypes and heart failure or rejection after heart transplantation. This may indicate that in heart transplantation, the total balance of cytokine production is more important for post-transplant rejection activities than the levels of IL-10 as such.",

keywords = "Gene Expression, Graft Rejection, Heart Failure, Heart Transplantation, Humans, Interleukin-10, Polymorphism, Single Nucleotide, Promoter Regions, Genetic",

author = "F.J. Bijlsma and A.H. Bruggink and M. Hartman and {Gmelig Meyling}, F.H.J. and M.G.J. Tilanus and {de Jonge}, N. and {de Weger}, R.A.",

year = "2001",

month = feb,

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volume = "57",

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journal = "Tissue Antigens",

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TY - JOUR

T1 - No association between IL-10 promoter gene polymorphism and heart failure or rejection following cardiac transplantation

AU - Bijlsma, F.J.

AU - Bruggink, A.H.

AU - Hartman, M.

AU - Gmelig Meyling, F.H.J.

AU - Tilanus, M.G.J.

AU - de Jonge, N.

AU - de Weger, R.A.

PY - 2001/2

Y1 - 2001/2

N2 - Expression of interleukin (IL)-10 influences the frequency of rejection events after organ transplantation. Therefore, 70 heart transplant patients were genotyped for three single nucleotide polymorphisms and a microsatellite polymorphism in the promotor region of the IL-10 gene. The promoter region was amplified by polymerase chain reaction and genotyped by a colorometric oligo ligation assay and gene scan analysis, respectively. Patient groups consisted of patients suffering from dilated cardiomyopathy or ischaemic heart disease. Cardiac donors served as control group. No correlation was found between genotypes and heart failure or rejection after heart transplantation. This may indicate that in heart transplantation, the total balance of cytokine production is more important for post-transplant rejection activities than the levels of IL-10 as such.

AB - Expression of interleukin (IL)-10 influences the frequency of rejection events after organ transplantation. Therefore, 70 heart transplant patients were genotyped for three single nucleotide polymorphisms and a microsatellite polymorphism in the promotor region of the IL-10 gene. The promoter region was amplified by polymerase chain reaction and genotyped by a colorometric oligo ligation assay and gene scan analysis, respectively. Patient groups consisted of patients suffering from dilated cardiomyopathy or ischaemic heart disease. Cardiac donors served as control group. No correlation was found between genotypes and heart failure or rejection after heart transplantation. This may indicate that in heart transplantation, the total balance of cytokine production is more important for post-transplant rejection activities than the levels of IL-10 as such.

KW - Gene Expression

KW - Graft Rejection

KW - Heart Failure

KW - Heart Transplantation

KW - Humans

KW - Interleukin-10

KW - Polymorphism, Single Nucleotide

KW - Promoter Regions, Genetic

M3 - Article

C2 - 11260510

SN - 0001-2815

VL - 57

SP - 151

EP - 153

JO - Tissue Antigens

JF - Tissue Antigens

IS - 2

ER -

No association between IL-10 promoter gene polymorphism and heart failure or rejection following cardiac transplantation

Abstract

Keywords

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