Neuronal expression of GFAP in patients with Alzheimer pathology and identification of novel GFAP splice forms

E.M. Hol; R.F. Roelofs; E. Moraal; M.A.F. Sonnemans; J.A. Sluijs; E.A. Proper; P.N.E. de Graan; D.F. Fischer; F.W. van Leeuwen

doi:10.1038/sj.mp.4001379

Neuronal expression of GFAP in patients with Alzheimer pathology and identification of novel GFAP splice forms

E.M. Hol, R.F. Roelofs, E. Moraal, M.A.F. Sonnemans, J.A. Sluijs, E.A. Proper, P.N.E. de Graan, D.F. Fischer, F.W. van Leeuwen

Translational Neuroscience

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Glial fibrillary acidic protein (GFAP) is considered to be a highly specific marker for glia. Here, we report on the expression of GFAP in neurons in the human hippocampus. Intriguingly, this neuronal GFAP is coded by out-of-frame splice variants and its expression is associated with Alzheimer pathology. We identified three novel GFAP splice forms: Delta 135 nt, Delta exon 6 and Delta 164 nt. Neuronal GFAP is mainly observed in the pyramidal neurons of the hippocampus of Alzheimer and Down syndrome patients and aged controls, but not in neurons of patients suffering from hippocampal sclerosis. Apparently, the hippocampal neurons in patients with Alzheimer's disease pathology are capable of expressing glia-specific genes.

Original language	English
Pages (from-to)	786-796
Number of pages	11
Journal	Molecular Psychiatry
Volume	8
Issue number	9
DOIs	https://doi.org/10.1038/sj.mp.4001379
Publication status	Published - Sept 2003

Keywords

Alternative Splicing
Alzheimer Disease
Amino Acid Sequence
Base Sequence
Down Syndrome
Epilepsy, Temporal Lobe
Exons
Female
Frameshifting, Ribosomal
Glial Fibrillary Acidic Protein
Hippocampus
Humans
Male
Molecular Sequence Data
Neuroglia
Neurons
RNA, Messenger
Reading Frames
Sclerosis
Transcription, Genetic
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

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10.1038/sj.mp.4001379

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title = "Neuronal expression of GFAP in patients with Alzheimer pathology and identification of novel GFAP splice forms",

abstract = "Glial fibrillary acidic protein (GFAP) is considered to be a highly specific marker for glia. Here, we report on the expression of GFAP in neurons in the human hippocampus. Intriguingly, this neuronal GFAP is coded by out-of-frame splice variants and its expression is associated with Alzheimer pathology. We identified three novel GFAP splice forms: Delta 135 nt, Delta exon 6 and Delta 164 nt. Neuronal GFAP is mainly observed in the pyramidal neurons of the hippocampus of Alzheimer and Down syndrome patients and aged controls, but not in neurons of patients suffering from hippocampal sclerosis. Apparently, the hippocampal neurons in patients with Alzheimer's disease pathology are capable of expressing glia-specific genes.",

keywords = "Alternative Splicing, Alzheimer Disease, Amino Acid Sequence, Base Sequence, Down Syndrome, Epilepsy, Temporal Lobe, Exons, Female, Frameshifting, Ribosomal, Glial Fibrillary Acidic Protein, Hippocampus, Humans, Male, Molecular Sequence Data, Neuroglia, Neurons, RNA, Messenger, Reading Frames, Sclerosis, Transcription, Genetic, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't",

author = "E.M. Hol and R.F. Roelofs and E. Moraal and M.A.F. Sonnemans and J.A. Sluijs and E.A. Proper and {de Graan}, P.N.E. and D.F. Fischer and {van Leeuwen}, F.W.",

year = "2003",

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doi = "10.1038/sj.mp.4001379",

language = "English",

volume = "8",

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AU - Hol, E.M.

AU - Roelofs, R.F.

AU - Moraal, E.

AU - Sonnemans, M.A.F.

AU - Sluijs, J.A.

AU - Proper, E.A.

AU - de Graan, P.N.E.

AU - Fischer, D.F.

AU - van Leeuwen, F.W.

PY - 2003/9

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N2 - Glial fibrillary acidic protein (GFAP) is considered to be a highly specific marker for glia. Here, we report on the expression of GFAP in neurons in the human hippocampus. Intriguingly, this neuronal GFAP is coded by out-of-frame splice variants and its expression is associated with Alzheimer pathology. We identified three novel GFAP splice forms: Delta 135 nt, Delta exon 6 and Delta 164 nt. Neuronal GFAP is mainly observed in the pyramidal neurons of the hippocampus of Alzheimer and Down syndrome patients and aged controls, but not in neurons of patients suffering from hippocampal sclerosis. Apparently, the hippocampal neurons in patients with Alzheimer's disease pathology are capable of expressing glia-specific genes.

AB - Glial fibrillary acidic protein (GFAP) is considered to be a highly specific marker for glia. Here, we report on the expression of GFAP in neurons in the human hippocampus. Intriguingly, this neuronal GFAP is coded by out-of-frame splice variants and its expression is associated with Alzheimer pathology. We identified three novel GFAP splice forms: Delta 135 nt, Delta exon 6 and Delta 164 nt. Neuronal GFAP is mainly observed in the pyramidal neurons of the hippocampus of Alzheimer and Down syndrome patients and aged controls, but not in neurons of patients suffering from hippocampal sclerosis. Apparently, the hippocampal neurons in patients with Alzheimer's disease pathology are capable of expressing glia-specific genes.

KW - Alternative Splicing

KW - Alzheimer Disease

KW - Amino Acid Sequence

KW - Base Sequence

KW - Down Syndrome

KW - Epilepsy, Temporal Lobe

KW - Exons

KW - Female

KW - Frameshifting, Ribosomal

KW - Glial Fibrillary Acidic Protein

KW - Hippocampus

KW - Humans

KW - Male

KW - Molecular Sequence Data

KW - Neuroglia

KW - Neurons

KW - RNA, Messenger

KW - Reading Frames

KW - Sclerosis

KW - Transcription, Genetic

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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DO - 10.1038/sj.mp.4001379

M3 - Article

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SN - 1359-4184

VL - 8

SP - 786

EP - 796

JO - Molecular Psychiatry

JF - Molecular Psychiatry

IS - 9

ER -

Neuronal expression of GFAP in patients with Alzheimer pathology and identification of novel GFAP splice forms

Abstract

Keywords

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