Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds

Kim Benders; W Boot; S.M. Cokelaere; P. René van Weeren; D Gawlitta; H.J. Bergman; DBF Saris; W.J.A. Dhert; J Malda

Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds

Kim Benders, W Boot, S.M. Cokelaere, P. René van Weeren, D Gawlitta, H.J. Bergman, DBF Saris, W.J.A. Dhert, J Malda

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

OBJECTIVE:

Although extracellular matrix (ECM)-derived scaffolds have been extensively studied and applied in a number of clinical applications, the use of ECM as a biomaterial for (osteo)chondral regeneration is less extensively explored. This study aimed at evaluating the chondrogenic potential of cells seeded on cartilage-derived matrix (CDM) scaffolds in vitro.

DESIGN:

Scaffolds were generated from decellularized equine articular cartilage and seeded with either chondrocytes or multipotent stromal cells (MSCs). After 2, 4, and 6 weeks of in vitro culture, CDM constructs were analyzed both histologically (hematoxylin and eosin, Safranin-O, collagen types I and II) and biochemically (glycosaminoglycan [GAG] and DNA content).

RESULTS:

After 4 weeks, both cell types demonstrated chondrogenic differentiation; however, the MSCs significantly outperformed chondrocytes in producing new GAG-containing cartilaginous matrix.

CONCLUSION:

These promising in vitro results underscore the potency of CDM scaffolds in (osteo)chondral defect repair.

Original language	English
Pages (from-to)	221-230
Journal	Cartilage
Volume	5
Issue number	4
Publication status	Published - Oct 2014

Cite this

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title = "Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds",

abstract = "OBJECTIVE: Although extracellular matrix (ECM)-derived scaffolds have been extensively studied and applied in a number of clinical applications, the use of ECM as a biomaterial for (osteo)chondral regeneration is less extensively explored. This study aimed at evaluating the chondrogenic potential of cells seeded on cartilage-derived matrix (CDM) scaffolds in vitro.DESIGN: Scaffolds were generated from decellularized equine articular cartilage and seeded with either chondrocytes or multipotent stromal cells (MSCs). After 2, 4, and 6 weeks of in vitro culture, CDM constructs were analyzed both histologically (hematoxylin and eosin, Safranin-O, collagen types I and II) and biochemically (glycosaminoglycan [GAG] and DNA content).RESULTS: After 4 weeks, both cell types demonstrated chondrogenic differentiation; however, the MSCs significantly outperformed chondrocytes in producing new GAG-containing cartilaginous matrix.CONCLUSION: These promising in vitro results underscore the potency of CDM scaffolds in (osteo)chondral defect repair.",

author = "Kim Benders and W Boot and S.M. Cokelaere and {van Weeren}, {P. Ren{\'e}} and D Gawlitta and H.J. Bergman and DBF Saris and W.J.A. Dhert and J Malda",

year = "2014",

month = oct,

language = "English",

volume = "5",

pages = "221--230",

journal = "Cartilage",

issn = "1947-6035",

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TY - JOUR

T1 - Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds

AU - Benders, Kim

AU - Boot, W

AU - Cokelaere, S.M.

AU - van Weeren, P. René

AU - Gawlitta, D

AU - Bergman, H.J.

AU - Saris, DBF

AU - Dhert, W.J.A.

AU - Malda, J

PY - 2014/10

Y1 - 2014/10

N2 - OBJECTIVE: Although extracellular matrix (ECM)-derived scaffolds have been extensively studied and applied in a number of clinical applications, the use of ECM as a biomaterial for (osteo)chondral regeneration is less extensively explored. This study aimed at evaluating the chondrogenic potential of cells seeded on cartilage-derived matrix (CDM) scaffolds in vitro.DESIGN: Scaffolds were generated from decellularized equine articular cartilage and seeded with either chondrocytes or multipotent stromal cells (MSCs). After 2, 4, and 6 weeks of in vitro culture, CDM constructs were analyzed both histologically (hematoxylin and eosin, Safranin-O, collagen types I and II) and biochemically (glycosaminoglycan [GAG] and DNA content).RESULTS: After 4 weeks, both cell types demonstrated chondrogenic differentiation; however, the MSCs significantly outperformed chondrocytes in producing new GAG-containing cartilaginous matrix.CONCLUSION: These promising in vitro results underscore the potency of CDM scaffolds in (osteo)chondral defect repair.

AB - OBJECTIVE: Although extracellular matrix (ECM)-derived scaffolds have been extensively studied and applied in a number of clinical applications, the use of ECM as a biomaterial for (osteo)chondral regeneration is less extensively explored. This study aimed at evaluating the chondrogenic potential of cells seeded on cartilage-derived matrix (CDM) scaffolds in vitro.DESIGN: Scaffolds were generated from decellularized equine articular cartilage and seeded with either chondrocytes or multipotent stromal cells (MSCs). After 2, 4, and 6 weeks of in vitro culture, CDM constructs were analyzed both histologically (hematoxylin and eosin, Safranin-O, collagen types I and II) and biochemically (glycosaminoglycan [GAG] and DNA content).RESULTS: After 4 weeks, both cell types demonstrated chondrogenic differentiation; however, the MSCs significantly outperformed chondrocytes in producing new GAG-containing cartilaginous matrix.CONCLUSION: These promising in vitro results underscore the potency of CDM scaffolds in (osteo)chondral defect repair.

M3 - Article

SN - 1947-6035

VL - 5

SP - 221

EP - 230

JO - Cartilage

JF - Cartilage

IS - 4

ER -

Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds

Abstract

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