Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds

Kim Benders, W Boot, S.M. Cokelaere, P. René van Weeren, D Gawlitta, H.J. Bergman, DBF Saris, W.J.A. Dhert, J Malda

    Research output: Contribution to journalArticleAcademicpeer-review



    Although extracellular matrix (ECM)-derived scaffolds have been extensively studied and applied in a number of clinical applications, the use of ECM as a biomaterial for (osteo)chondral regeneration is less extensively explored. This study aimed at evaluating the chondrogenic potential of cells seeded on cartilage-derived matrix (CDM) scaffolds in vitro.


    Scaffolds were generated from decellularized equine articular cartilage and seeded with either chondrocytes or multipotent stromal cells (MSCs). After 2, 4, and 6 weeks of in vitro culture, CDM constructs were analyzed both histologically (hematoxylin and eosin, Safranin-O, collagen types I and II) and biochemically (glycosaminoglycan [GAG] and DNA content).


    After 4 weeks, both cell types demonstrated chondrogenic differentiation; however, the MSCs significantly outperformed chondrocytes in producing new GAG-containing cartilaginous matrix.


    These promising in vitro results underscore the potency of CDM scaffolds in (osteo)chondral defect repair.
    Original languageEnglish
    Pages (from-to)221-230
    Issue number4
    Publication statusPublished - Oct 2014


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