Multicenter evaluation of a rapid electrochemiluminescent adrenocorticotropic hormone (ACTH) immunoassay

Inge Verschraegen; Ellen Anckaert; Johan Schiettecatte; Marleen Mees; Adolfo Garrido; Derik Hermsen; Eef G W M Lentjes; Andreas Liebert; Heinz-Jürgen Roth; Gudrun Stamminger; Johan Smitz

doi:10.1016/j.cca.2006.12.031

Multicenter evaluation of a rapid electrochemiluminescent adrenocorticotropic hormone (ACTH) immunoassay

Inge Verschraegen, Ellen Anckaert^*, Johan Schiettecatte, Marleen Mees, Adolfo Garrido, Derik Hermsen, Eef G W M Lentjes, Andreas Liebert, Heinz-Jürgen Roth, Gudrun Stamminger, Johan Smitz

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Measuring plasma adrenocorticotropic hormone (ACTH) is a key step in the differential diagnosis of hypothalamic-pituitary-adrenal disorders.

METHODS: The recently developed electrochemiluminescence Elecsys ACTH immunoassay (Roche Diagnostics, Mannheim, Germany) was evaluated at six clinical laboratories on the Modular E170 and/or the Elecsys 2010 (Roche Diagnostics) immunoanalysers.

RESULTS: The within-run and between-run imprecision was <or=1.9% and <or=5%, respectively (concentration level 12-971 ng/L). Functional sensitivity was at least 1.8 ng/L. The Elecsys assay correlated well with two established ACTH immunoradiometric assays (Brahms ACTH RIA and Nichols ACTH IRMA) and with the Immulite ACTH assay. Reference values for the Elecsys ACTH assay, determined as the percentile 5 to 95 (2.5 to 97.5) interval in 354 adults without disorder with impact on the hypothalamic-pituitary-adrenal axis, were between 7.2 and 63 ng/L (5.6 and 76 ng/L). The clinical sensitivity of the Elecsys ACTH assay in 57 patients with confirmed disorders of the pituitary-adrenal axis was comparable to the established Brahms ACTH assay.

CONCLUSION: The non-isotopic Elecsys ACTH immunoassay provides precision and reliability in combination with reduced turnaround time.

Original language	English
Pages (from-to)	75-80
Number of pages	6
Journal	Clinica Chimica Acta
Volume	380
Issue number	1-2
DOIs	https://doi.org/10.1016/j.cca.2006.12.031
Publication status	Published - 1 May 2007

Keywords

Adrenal Gland Diseases/blood
Adrenocorticotropic Hormone/analysis
Adult
Aged
Aged, 80 and over
Female
Humans
Immunoassay/instrumentation
Luminescent Measurements/instrumentation
Male
Middle Aged
Pituitary Diseases/blood
Pituitary-Adrenal System/metabolism
Reference Values
Sensitivity and Specificity

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10.1016/j.cca.2006.12.031

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title = "Multicenter evaluation of a rapid electrochemiluminescent adrenocorticotropic hormone (ACTH) immunoassay",

abstract = "BACKGROUND: Measuring plasma adrenocorticotropic hormone (ACTH) is a key step in the differential diagnosis of hypothalamic-pituitary-adrenal disorders.METHODS: The recently developed electrochemiluminescence Elecsys ACTH immunoassay (Roche Diagnostics, Mannheim, Germany) was evaluated at six clinical laboratories on the Modular E170 and/or the Elecsys 2010 (Roche Diagnostics) immunoanalysers.RESULTS: The within-run and between-run imprecision was CONCLUSION: The non-isotopic Elecsys ACTH immunoassay provides precision and reliability in combination with reduced turnaround time.",

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author = "Inge Verschraegen and Ellen Anckaert and Johan Schiettecatte and Marleen Mees and Adolfo Garrido and Derik Hermsen and Lentjes, {Eef G W M} and Andreas Liebert and Heinz-J{\"u}rgen Roth and Gudrun Stamminger and Johan Smitz",

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doi = "10.1016/j.cca.2006.12.031",

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T1 - Multicenter evaluation of a rapid electrochemiluminescent adrenocorticotropic hormone (ACTH) immunoassay

AU - Verschraegen, Inge

AU - Anckaert, Ellen

AU - Schiettecatte, Johan

AU - Mees, Marleen

AU - Garrido, Adolfo

AU - Hermsen, Derik

AU - Lentjes, Eef G W M

AU - Liebert, Andreas

AU - Roth, Heinz-Jürgen

AU - Stamminger, Gudrun

AU - Smitz, Johan

PY - 2007/5/1

Y1 - 2007/5/1

N2 - BACKGROUND: Measuring plasma adrenocorticotropic hormone (ACTH) is a key step in the differential diagnosis of hypothalamic-pituitary-adrenal disorders.METHODS: The recently developed electrochemiluminescence Elecsys ACTH immunoassay (Roche Diagnostics, Mannheim, Germany) was evaluated at six clinical laboratories on the Modular E170 and/or the Elecsys 2010 (Roche Diagnostics) immunoanalysers.RESULTS: The within-run and between-run imprecision was CONCLUSION: The non-isotopic Elecsys ACTH immunoassay provides precision and reliability in combination with reduced turnaround time.

AB - BACKGROUND: Measuring plasma adrenocorticotropic hormone (ACTH) is a key step in the differential diagnosis of hypothalamic-pituitary-adrenal disorders.METHODS: The recently developed electrochemiluminescence Elecsys ACTH immunoassay (Roche Diagnostics, Mannheim, Germany) was evaluated at six clinical laboratories on the Modular E170 and/or the Elecsys 2010 (Roche Diagnostics) immunoanalysers.RESULTS: The within-run and between-run imprecision was CONCLUSION: The non-isotopic Elecsys ACTH immunoassay provides precision and reliability in combination with reduced turnaround time.

KW - Adrenal Gland Diseases/blood

KW - Adrenocorticotropic Hormone/analysis

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Female

KW - Humans

KW - Immunoassay/instrumentation

KW - Luminescent Measurements/instrumentation

KW - Male

KW - Middle Aged

KW - Pituitary Diseases/blood

KW - Pituitary-Adrenal System/metabolism

KW - Reference Values

KW - Sensitivity and Specificity

U2 - 10.1016/j.cca.2006.12.031

DO - 10.1016/j.cca.2006.12.031

M3 - Article

C2 - 17321508

SN - 0009-8981

VL - 380

SP - 75

EP - 80

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 1-2

ER -

Multicenter evaluation of a rapid electrochemiluminescent adrenocorticotropic hormone (ACTH) immunoassay

Abstract

Keywords

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