Monoclonal cell surface antibodies do not produce short-term effects on electrical properties of mouse oligodendrocytes in culture

H. Kettenmann; I. Sommer; M. Schachner

doi:10.1016/S0304-3940(85)80078-1

Monoclonal cell surface antibodies do not produce short-term effects on electrical properties of mouse oligodendrocytes in culture

H. Kettenmann^*, I. Sommer, M. Schachner

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

Eleven monoclonal antibodies (O₁–O₁₁) directed against the surface of oligodendrocytes were applied individually or in combination during measurement of membrane potential, input resistance and K⁺-pump activity in explant cultures of mouse spinal cord. Antibody binding to oligodendrocytes was verified by indirect immunofluorescence. None of the antibodies affected the electrical properties studied. On the basis of these observations, it is possible to identify oligodendrocytes immunocytologically prior to electrophysiological characterization.

Original language	English
Pages (from-to)	195-199
Number of pages	5
Journal	Neuroscience Letters
Volume	54
Issue number	2-3
DOIs	https://doi.org/10.1016/S0304-3940(85)80078-1
Publication status	Published - 1 Jan 1985

Keywords

cell culture
cell surface antigens
cell type-specific monoclonal antibodies
glia
membrane properties
mouse
oligodendrocyte

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10.1016/S0304-3940(85)80078-1

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abstract = "Eleven monoclonal antibodies (O1–O11) directed against the surface of oligodendrocytes were applied individually or in combination during measurement of membrane potential, input resistance and K+-pump activity in explant cultures of mouse spinal cord. Antibody binding to oligodendrocytes was verified by indirect immunofluorescence. None of the antibodies affected the electrical properties studied. On the basis of these observations, it is possible to identify oligodendrocytes immunocytologically prior to electrophysiological characterization.",

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AU - Sommer, I.

AU - Schachner, M.

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N2 - Eleven monoclonal antibodies (O1–O11) directed against the surface of oligodendrocytes were applied individually or in combination during measurement of membrane potential, input resistance and K+-pump activity in explant cultures of mouse spinal cord. Antibody binding to oligodendrocytes was verified by indirect immunofluorescence. None of the antibodies affected the electrical properties studied. On the basis of these observations, it is possible to identify oligodendrocytes immunocytologically prior to electrophysiological characterization.

AB - Eleven monoclonal antibodies (O1–O11) directed against the surface of oligodendrocytes were applied individually or in combination during measurement of membrane potential, input resistance and K+-pump activity in explant cultures of mouse spinal cord. Antibody binding to oligodendrocytes was verified by indirect immunofluorescence. None of the antibodies affected the electrical properties studied. On the basis of these observations, it is possible to identify oligodendrocytes immunocytologically prior to electrophysiological characterization.

KW - cell culture

KW - cell surface antigens

KW - cell type-specific monoclonal antibodies

KW - glia

KW - membrane properties

KW - mouse

KW - oligodendrocyte

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EP - 199

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2-3

ER -

Monoclonal cell surface antibodies do not produce short-term effects on electrical properties of mouse oligodendrocytes in culture

Abstract

Keywords

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