Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer

A P M Jongsma; Jurgen M J Piek; RP Zweemer; RHM Verheijen; J W T Klein Gebbinck; G.J. Kamp; I.J. Jacobs; P. Shaw; P. J.  van Diest; P. Kenemans

doi:10.1136/mp.55.5.305

Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer

A P M Jongsma, Jurgen M J Piek, RP Zweemer, RHM Verheijen, J W T Klein Gebbinck, G.J. Kamp, I.J. Jacobs, P. Shaw, P. J. van Diest, P. Kenemans

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND/AIMS: Loss of heterozygosity (LOH) on chromosome 13q has been reported to occur frequently in human ovarian cancer, and indications have been found that chromosome 13 may also play a specific role in the inherited form of ovarian cancer. The aim of this study was to define regions on chromosome 13 that may harbour additional tumour suppressor genes involved in the tumorigenesis of BRCA1 related ovarian and fallopian tube cancer.

MATERIALS/METHODS: DNA extracted from paraffin wax blocks of 36 BRCA1 associated ovarian and fallopian tube carcinomas was analysed by LOH polymerase chain reaction using seven highly polymorphic microsatellite markers spanning chromosome 13q.

RESULTS: High LOH frequencies were found on loci 13q11, 13q14, 13q21, 13q22-31, 13q32, and 13q32-4, suggesting the presence of putative tumour suppressor genes on the long arm of chromosome 13 that may play a role in the pathogenesis of BRCA1 related ovarian and fallopian tube cancer. LOH patterns appeared to be independent of the type of BRCA1 mutation, stage, and grade. Although in some cases there were indications for loss of larger parts of chromosome 13, in most cases losses were fairly randomly distributed over chromosome 13 with retained parts in between lost parts. Microsatellite instability was found in six cases.

CONCLUSION: Several loci on chromosome 13q show high frequencies of LOH in BRCA1 related ovarian and fallopian tube cancer, and may therefore harbour putative tumour suppressor genes involved in the carcinogenesis of this particular type of hereditary cancer.

Original language	English
Pages (from-to)	305-309
Number of pages	5
Journal	Molecular Pathology : MP
Volume	55
Issue number	5
DOIs	https://doi.org/10.1136/mp.55.5.305
Publication status	Published - 2002
Externally published	Yes

Keywords

Chromosomes, Human, Pair 13
DNA, Neoplasm
Fallopian Tube Neoplasms
Female
Genes, BRCA1
Genes, Tumor Suppressor
Humans
Loss of Heterozygosity
Microsatellite Repeats
Neoplasm Staging
Ovarian Neoplasms
Polymerase Chain Reaction

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10.1136/mp.55.5.305

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Jongsma, A. P. M., Piek, J. M. J., Zweemer, RP., Verheijen, RHM., Klein Gebbinck, J. W. T., Kamp, G. J., Jacobs, I. J., Shaw, P., van Diest, P. J., & Kenemans, P. (2002). Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer. Molecular Pathology : MP, 55(5), 305-309. https://doi.org/10.1136/mp.55.5.305

@article{43f77db765224976a4e5b84ad9c76afc,

title = "Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer",

abstract = "BACKGROUND/AIMS: Loss of heterozygosity (LOH) on chromosome 13q has been reported to occur frequently in human ovarian cancer, and indications have been found that chromosome 13 may also play a specific role in the inherited form of ovarian cancer. The aim of this study was to define regions on chromosome 13 that may harbour additional tumour suppressor genes involved in the tumorigenesis of BRCA1 related ovarian and fallopian tube cancer.MATERIALS/METHODS: DNA extracted from paraffin wax blocks of 36 BRCA1 associated ovarian and fallopian tube carcinomas was analysed by LOH polymerase chain reaction using seven highly polymorphic microsatellite markers spanning chromosome 13q.RESULTS: High LOH frequencies were found on loci 13q11, 13q14, 13q21, 13q22-31, 13q32, and 13q32-4, suggesting the presence of putative tumour suppressor genes on the long arm of chromosome 13 that may play a role in the pathogenesis of BRCA1 related ovarian and fallopian tube cancer. LOH patterns appeared to be independent of the type of BRCA1 mutation, stage, and grade. Although in some cases there were indications for loss of larger parts of chromosome 13, in most cases losses were fairly randomly distributed over chromosome 13 with retained parts in between lost parts. Microsatellite instability was found in six cases.CONCLUSION: Several loci on chromosome 13q show high frequencies of LOH in BRCA1 related ovarian and fallopian tube cancer, and may therefore harbour putative tumour suppressor genes involved in the carcinogenesis of this particular type of hereditary cancer.",

keywords = "Chromosomes, Human, Pair 13, DNA, Neoplasm, Fallopian Tube Neoplasms, Female, Genes, BRCA1, Genes, Tumor Suppressor, Humans, Loss of Heterozygosity, Microsatellite Repeats, Neoplasm Staging, Ovarian Neoplasms, Polymerase Chain Reaction",

author = "Jongsma, {A P M} and Piek, {Jurgen M J} and RP Zweemer and RHM Verheijen and {Klein Gebbinck}, {J W T} and G.J. Kamp and I.J. Jacobs and P. Shaw and {van Diest}, {P. J.} and P. Kenemans",

year = "2002",

doi = "10.1136/mp.55.5.305",

language = "English",

volume = "55",

pages = "305--309",

journal = "Molecular Pathology : MP",

issn = "1366-8714",

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T1 - Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer

AU - Jongsma, A P M

AU - Piek, Jurgen M J

AU - Zweemer, RP

AU - Verheijen, RHM

AU - Klein Gebbinck, J W T

AU - Kamp, G.J.

AU - Jacobs, I.J.

AU - Shaw, P.

AU - van Diest, P. J.

AU - Kenemans, P.

PY - 2002

Y1 - 2002

N2 - BACKGROUND/AIMS: Loss of heterozygosity (LOH) on chromosome 13q has been reported to occur frequently in human ovarian cancer, and indications have been found that chromosome 13 may also play a specific role in the inherited form of ovarian cancer. The aim of this study was to define regions on chromosome 13 that may harbour additional tumour suppressor genes involved in the tumorigenesis of BRCA1 related ovarian and fallopian tube cancer.MATERIALS/METHODS: DNA extracted from paraffin wax blocks of 36 BRCA1 associated ovarian and fallopian tube carcinomas was analysed by LOH polymerase chain reaction using seven highly polymorphic microsatellite markers spanning chromosome 13q.RESULTS: High LOH frequencies were found on loci 13q11, 13q14, 13q21, 13q22-31, 13q32, and 13q32-4, suggesting the presence of putative tumour suppressor genes on the long arm of chromosome 13 that may play a role in the pathogenesis of BRCA1 related ovarian and fallopian tube cancer. LOH patterns appeared to be independent of the type of BRCA1 mutation, stage, and grade. Although in some cases there were indications for loss of larger parts of chromosome 13, in most cases losses were fairly randomly distributed over chromosome 13 with retained parts in between lost parts. Microsatellite instability was found in six cases.CONCLUSION: Several loci on chromosome 13q show high frequencies of LOH in BRCA1 related ovarian and fallopian tube cancer, and may therefore harbour putative tumour suppressor genes involved in the carcinogenesis of this particular type of hereditary cancer.

AB - BACKGROUND/AIMS: Loss of heterozygosity (LOH) on chromosome 13q has been reported to occur frequently in human ovarian cancer, and indications have been found that chromosome 13 may also play a specific role in the inherited form of ovarian cancer. The aim of this study was to define regions on chromosome 13 that may harbour additional tumour suppressor genes involved in the tumorigenesis of BRCA1 related ovarian and fallopian tube cancer.MATERIALS/METHODS: DNA extracted from paraffin wax blocks of 36 BRCA1 associated ovarian and fallopian tube carcinomas was analysed by LOH polymerase chain reaction using seven highly polymorphic microsatellite markers spanning chromosome 13q.RESULTS: High LOH frequencies were found on loci 13q11, 13q14, 13q21, 13q22-31, 13q32, and 13q32-4, suggesting the presence of putative tumour suppressor genes on the long arm of chromosome 13 that may play a role in the pathogenesis of BRCA1 related ovarian and fallopian tube cancer. LOH patterns appeared to be independent of the type of BRCA1 mutation, stage, and grade. Although in some cases there were indications for loss of larger parts of chromosome 13, in most cases losses were fairly randomly distributed over chromosome 13 with retained parts in between lost parts. Microsatellite instability was found in six cases.CONCLUSION: Several loci on chromosome 13q show high frequencies of LOH in BRCA1 related ovarian and fallopian tube cancer, and may therefore harbour putative tumour suppressor genes involved in the carcinogenesis of this particular type of hereditary cancer.

KW - Chromosomes, Human, Pair 13

KW - DNA, Neoplasm

KW - Fallopian Tube Neoplasms

KW - Female

KW - Genes, BRCA1

KW - Genes, Tumor Suppressor

KW - Humans

KW - Loss of Heterozygosity

KW - Microsatellite Repeats

KW - Neoplasm Staging

KW - Ovarian Neoplasms

KW - Polymerase Chain Reaction

U2 - 10.1136/mp.55.5.305

DO - 10.1136/mp.55.5.305

M3 - Article

C2 - 12354934

SN - 1366-8714

VL - 55

SP - 305

EP - 309

JO - Molecular Pathology : MP

JF - Molecular Pathology : MP

IS - 5

ER -

Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer

Abstract

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