TY - JOUR
T1 - Molecular characterization of mycobacterium tuberculosis strains with TB-SPRINT
AU - Molina-Moya, Barbara
AU - Gomgnimbou, Michel Kiréopori
AU - Lafoz, Carmen
AU - Lacoma, Alicia
AU - Prat, Cristina
AU - Refrégier, Guislaine
AU - Samper, Sofia
AU - Dominguez, Jose
AU - Sola, Christophe
N1 - Funding Information:
Financial support: The research was supported by grants from the Instituto de Salud Carlos III (PI07/0551, PI13/0154, PI15/0317, and PI16/01912).
Publisher Copyright:
© Copyright 2017 by The American Society of Tropical Medicine and Hygiene.
PY - 2017
Y1 - 2017
N2 - We evaluated Tuberculosis-Spoligo-Rifampicin-Isoniazid Typing (TB-SPRINT), a microbead-based method for spoligotyping and detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis. For that, 67 M. tuberculosis complex strains were retrospectively selected. Membrane-based spoligotyping, restriction fragment length polymorphism, DNA sequencing/pyrosequencing of rpoB, katG, and inhA promoter, TB-SPRINT, and SNP typing were performed. Concordance between spoligotyping methods was 99.6% (2,785/2,795 spoligotype data points). For most of the discordant cases, the same lineage was assigned with both methods. Concordance between phenotypic drug susceptibility testing and TB-SPRINT for detecting rifampicin and isoniazid resistance was 98.4% (63/64) and 93.8% (60/64), respectively. Concordance between DNA sequencing/pyrosequencing and TB-SPRINT for detecting mutations inrpoB, katG, and inhA were 98.4%(60/61),100%(64/64), and 96.9% (62/64), respectively. Inconclusion, TB-SPRINTisa rapid and easy-to-perform assay for genotyping and detecting drug resistance in a single tube; therefore, it may be a useful tool to improve epidemiological surveillance.
AB - We evaluated Tuberculosis-Spoligo-Rifampicin-Isoniazid Typing (TB-SPRINT), a microbead-based method for spoligotyping and detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis. For that, 67 M. tuberculosis complex strains were retrospectively selected. Membrane-based spoligotyping, restriction fragment length polymorphism, DNA sequencing/pyrosequencing of rpoB, katG, and inhA promoter, TB-SPRINT, and SNP typing were performed. Concordance between spoligotyping methods was 99.6% (2,785/2,795 spoligotype data points). For most of the discordant cases, the same lineage was assigned with both methods. Concordance between phenotypic drug susceptibility testing and TB-SPRINT for detecting rifampicin and isoniazid resistance was 98.4% (63/64) and 93.8% (60/64), respectively. Concordance between DNA sequencing/pyrosequencing and TB-SPRINT for detecting mutations inrpoB, katG, and inhA were 98.4%(60/61),100%(64/64), and 96.9% (62/64), respectively. Inconclusion, TB-SPRINTisa rapid and easy-to-perform assay for genotyping and detecting drug resistance in a single tube; therefore, it may be a useful tool to improve epidemiological surveillance.
UR - http://www.scopus.com/inward/record.url?scp=85028955273&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.16-0782
DO - 10.4269/ajtmh.16-0782
M3 - Article
C2 - 28722603
AN - SCOPUS:85028955273
SN - 0002-9637
VL - 97
SP - 806
EP - 809
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 3
ER -