Molecular characterization and T-cell-stimulatory capacity of Mycobacterium leprae antigen T5

B. Wieles; E. Spierings; J. Van Noort; B. Naafs; R. Offringa; T. Ottenhoff

Molecular characterization and T-cell-stimulatory capacity of Mycobacterium leprae antigen T5

B. Wieles^*, E. Spierings, J. Van Noort, B. Naafs, R. Offringa, T. Ottenhoff

^*Corresponding author for this work

Infection & Immunity

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

A Mycobacterium leprae χt11 clone designated T5 has previously been selected with sera from tuberculoid leprosy patients. Sequence analysis of this clone revealed the presence of two overlapping open reading frames (ORFs) present on the two cDNA strands. The first ORF codes for the serologically recognized antigen, which was fused with the lacZ gene in the λgt11 clone. The second ORF, present on the complementary strand, displays strong sequence homology with the aspartyl-tRNA synthetase genes of Escherichia coli and Thermus thermophilus. Here we show that the purified T5- derived product, overexpressed in E. coli, is recognized by T cells of the majority of the leprosy patients tested, including lepromatous leprosy patients who do not respond to whole M. leprae bacilli.

Original language	English
Pages (from-to)	4682-4685
Number of pages	4
Journal	Infection and Immunity
Volume	63
Issue number	12
Publication status	Published - 1995

Cite this

@article{3718f7f603d14741883aad7dc43ac99e,

title = "Molecular characterization and T-cell-stimulatory capacity of Mycobacterium leprae antigen T5",

abstract = "A Mycobacterium leprae χt11 clone designated T5 has previously been selected with sera from tuberculoid leprosy patients. Sequence analysis of this clone revealed the presence of two overlapping open reading frames (ORFs) present on the two cDNA strands. The first ORF codes for the serologically recognized antigen, which was fused with the lacZ gene in the λgt11 clone. The second ORF, present on the complementary strand, displays strong sequence homology with the aspartyl-tRNA synthetase genes of Escherichia coli and Thermus thermophilus. Here we show that the purified T5- derived product, overexpressed in E. coli, is recognized by T cells of the majority of the leprosy patients tested, including lepromatous leprosy patients who do not respond to whole M. leprae bacilli.",

author = "B. Wieles and E. Spierings and {Van Noort}, J. and B. Naafs and R. Offringa and T. Ottenhoff",

year = "1995",

language = "English",

volume = "63",

pages = "4682--4685",

journal = "Infection and Immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

number = "12",

}

TY - JOUR

T1 - Molecular characterization and T-cell-stimulatory capacity of Mycobacterium leprae antigen T5

AU - Wieles, B.

AU - Spierings, E.

AU - Van Noort, J.

AU - Naafs, B.

AU - Offringa, R.

AU - Ottenhoff, T.

PY - 1995

Y1 - 1995

N2 - A Mycobacterium leprae χt11 clone designated T5 has previously been selected with sera from tuberculoid leprosy patients. Sequence analysis of this clone revealed the presence of two overlapping open reading frames (ORFs) present on the two cDNA strands. The first ORF codes for the serologically recognized antigen, which was fused with the lacZ gene in the λgt11 clone. The second ORF, present on the complementary strand, displays strong sequence homology with the aspartyl-tRNA synthetase genes of Escherichia coli and Thermus thermophilus. Here we show that the purified T5- derived product, overexpressed in E. coli, is recognized by T cells of the majority of the leprosy patients tested, including lepromatous leprosy patients who do not respond to whole M. leprae bacilli.

AB - A Mycobacterium leprae χt11 clone designated T5 has previously been selected with sera from tuberculoid leprosy patients. Sequence analysis of this clone revealed the presence of two overlapping open reading frames (ORFs) present on the two cDNA strands. The first ORF codes for the serologically recognized antigen, which was fused with the lacZ gene in the λgt11 clone. The second ORF, present on the complementary strand, displays strong sequence homology with the aspartyl-tRNA synthetase genes of Escherichia coli and Thermus thermophilus. Here we show that the purified T5- derived product, overexpressed in E. coli, is recognized by T cells of the majority of the leprosy patients tested, including lepromatous leprosy patients who do not respond to whole M. leprae bacilli.

UR - http://www.scopus.com/inward/record.url?scp=0028884284&partnerID=8YFLogxK

M3 - Article

C2 - 7591123

AN - SCOPUS:0028884284

SN - 0019-9567

VL - 63

SP - 4682

EP - 4685

JO - Infection and Immunity

JF - Infection and Immunity

IS - 12

ER -

Molecular characterization and T-cell-stimulatory capacity of Mycobacterium leprae antigen T5

Abstract

Other files and links

Fingerprint

Cite this