Modulation of DNA topoisomerase II activity and expression in melanoma cells with acquired drug resistance

H Lage; H Helmbach; M Dietel; D Schadendorf

doi:10.1054/bjoc.1999.0947

Modulation of DNA topoisomerase II activity and expression in melanoma cells with acquired drug resistance

H Lage, H Helmbach, M Dietel, D Schadendorf

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The role of DNA topoisomerases (Topo) IIalpha and IIbeta was investigated in various drug-resistant melanoma cells. Melanoma cells resistant to etoposide, exhibited an up to tenfold reduced Topo II activity corresponding to an increasing degree of drug resistance indicating that modulation of Topo II activity contribute to the drug-resistant phenotype. The reduction of Topo II activity was reflected by decreased nuclear amounts of both Topo II isoforms.

Original language	English
Pages (from-to)	488-91
Number of pages	4
Journal	British Journal of Cancer
Volume	82
Issue number	2
DOIs	https://doi.org/10.1054/bjoc.1999.0947
Publication status	Published - Jan 2000

Keywords

Antineoplastic Agents, Phytogenic
Base Sequence
DNA Primers
DNA Topoisomerases, Type II
Drug Resistance, Neoplasm
Etoposide
Humans
Melanoma
Molecular Sequence Data
Protein Isoforms
Tumor Cells, Cultured

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10.1054/bjoc.1999.0947

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title = "Modulation of DNA topoisomerase II activity and expression in melanoma cells with acquired drug resistance",

abstract = "The role of DNA topoisomerases (Topo) IIalpha and IIbeta was investigated in various drug-resistant melanoma cells. Melanoma cells resistant to etoposide, exhibited an up to tenfold reduced Topo II activity corresponding to an increasing degree of drug resistance indicating that modulation of Topo II activity contribute to the drug-resistant phenotype. The reduction of Topo II activity was reflected by decreased nuclear amounts of both Topo II isoforms.",

keywords = "Antineoplastic Agents, Phytogenic, Base Sequence, DNA Primers, DNA Topoisomerases, Type II, Drug Resistance, Neoplasm, Etoposide, Humans, Melanoma, Molecular Sequence Data, Protein Isoforms, Tumor Cells, Cultured",

author = "H Lage and H Helmbach and M Dietel and D Schadendorf",

year = "2000",

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doi = "10.1054/bjoc.1999.0947",

language = "English",

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T1 - Modulation of DNA topoisomerase II activity and expression in melanoma cells with acquired drug resistance

AU - Lage, H

AU - Helmbach, H

AU - Dietel, M

AU - Schadendorf, D

PY - 2000/1

Y1 - 2000/1

N2 - The role of DNA topoisomerases (Topo) IIalpha and IIbeta was investigated in various drug-resistant melanoma cells. Melanoma cells resistant to etoposide, exhibited an up to tenfold reduced Topo II activity corresponding to an increasing degree of drug resistance indicating that modulation of Topo II activity contribute to the drug-resistant phenotype. The reduction of Topo II activity was reflected by decreased nuclear amounts of both Topo II isoforms.

AB - The role of DNA topoisomerases (Topo) IIalpha and IIbeta was investigated in various drug-resistant melanoma cells. Melanoma cells resistant to etoposide, exhibited an up to tenfold reduced Topo II activity corresponding to an increasing degree of drug resistance indicating that modulation of Topo II activity contribute to the drug-resistant phenotype. The reduction of Topo II activity was reflected by decreased nuclear amounts of both Topo II isoforms.

KW - Antineoplastic Agents, Phytogenic

KW - Base Sequence

KW - DNA Primers

KW - DNA Topoisomerases, Type II

KW - Drug Resistance, Neoplasm

KW - Etoposide

KW - Humans

KW - Melanoma

KW - Molecular Sequence Data

KW - Protein Isoforms

KW - Tumor Cells, Cultured

U2 - 10.1054/bjoc.1999.0947

DO - 10.1054/bjoc.1999.0947

M3 - Article

C2 - 10646909

SN - 0007-0920

VL - 82

SP - 488

EP - 491

JO - British Journal of Cancer

JF - British Journal of Cancer

IS - 2

ER -

Modulation of DNA topoisomerase II activity and expression in melanoma cells with acquired drug resistance

Abstract

Keywords

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