Modeling Human Digestive Diseases With CRISPR-Cas9–Modified Organoids

Masayuki Fujii; Hans Clevers; Toshiro Sato

doi:10.1053/j.gastro.2018.11.048

Modeling Human Digestive Diseases With CRISPR-Cas9–Modified Organoids

Masayuki Fujii, Hans Clevers, Toshiro Sato^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

8 Citations (Scopus)

Abstract

Insights into the stem cell niche have allowed researchers to cultivate adult tissue stem cells as organoids that display structural and phenotypic features of healthy and diseased epithelial tissues. Organoids derived from patients’ tissues are used as models of disease and to test drugs. CRISPR-Cas9 technology can be used to genetically engineer organoids for studies of monogenic diseases and cancer. We review the derivation of organoids from human gastrointestinal tissues and how CRISPR-Cas9 technology can be used to study these organoids. We discuss burgeoning technologies that are broadening our understanding of diseases of the digestive system.

Original language	English
Pages (from-to)	562-576
Number of pages	15
Journal	Gastroenterology
Volume	156
Issue number	3
DOIs	https://doi.org/10.1053/j.gastro.2018.11.048
Publication status	Published - 1 Feb 2019

Keywords

Colorectal Cancer
Gastric Cancer
Inflammatory Bowel Disease
Pancreatic Cancer

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10.1053/j.gastro.2018.11.048

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title = "Modeling Human Digestive Diseases With CRISPR-Cas9–Modified Organoids",

abstract = "Insights into the stem cell niche have allowed researchers to cultivate adult tissue stem cells as organoids that display structural and phenotypic features of healthy and diseased epithelial tissues. Organoids derived from patients{\textquoteright} tissues are used as models of disease and to test drugs. CRISPR-Cas9 technology can be used to genetically engineer organoids for studies of monogenic diseases and cancer. We review the derivation of organoids from human gastrointestinal tissues and how CRISPR-Cas9 technology can be used to study these organoids. We discuss burgeoning technologies that are broadening our understanding of diseases of the digestive system.",

keywords = "Colorectal Cancer, Gastric Cancer, Inflammatory Bowel Disease, Pancreatic Cancer",

author = "Masayuki Fujii and Hans Clevers and Toshiro Sato",

note = "Funding Information: Funding M.F. is supported by the Japan Society for the Promotion of Science Research Fellowships for Young Scientists. Publisher Copyright: {\textcopyright} 2019 AGA Institute",

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AU - Fujii, Masayuki

AU - Clevers, Hans

AU - Sato, Toshiro

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Insights into the stem cell niche have allowed researchers to cultivate adult tissue stem cells as organoids that display structural and phenotypic features of healthy and diseased epithelial tissues. Organoids derived from patients’ tissues are used as models of disease and to test drugs. CRISPR-Cas9 technology can be used to genetically engineer organoids for studies of monogenic diseases and cancer. We review the derivation of organoids from human gastrointestinal tissues and how CRISPR-Cas9 technology can be used to study these organoids. We discuss burgeoning technologies that are broadening our understanding of diseases of the digestive system.

AB - Insights into the stem cell niche have allowed researchers to cultivate adult tissue stem cells as organoids that display structural and phenotypic features of healthy and diseased epithelial tissues. Organoids derived from patients’ tissues are used as models of disease and to test drugs. CRISPR-Cas9 technology can be used to genetically engineer organoids for studies of monogenic diseases and cancer. We review the derivation of organoids from human gastrointestinal tissues and how CRISPR-Cas9 technology can be used to study these organoids. We discuss burgeoning technologies that are broadening our understanding of diseases of the digestive system.

KW - Colorectal Cancer

KW - Gastric Cancer

KW - Inflammatory Bowel Disease

KW - Pancreatic Cancer

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DO - 10.1053/j.gastro.2018.11.048

M3 - Review article

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SN - 0016-5085

VL - 156

SP - 562

EP - 576

JO - Gastroenterology

JF - Gastroenterology

IS - 3

ER -

Modeling Human Digestive Diseases With CRISPR-Cas9–Modified Organoids

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