Abstract
This paper describes the kinetics of fibroblastic colony forming units (CFU-f) in murine blood after phenylhydrazine-induced haemolytic anaemia and their subsequent migration into haemopoietic organs. Murine blood contained 5.3 +/- 0.8 CFU-f per 10(6) nucleated cells. Absence of particle ingestion and factor VIII-related antigen in addition to the enzyme pattern in CFU-f-derived cells confirmed that these cells did not have a macrophage-like or endothelial nature. Phenylhydrazine treatment of mice resulted in a 3-fold increase in blood CFU-f numbers which was accompanied by increases in blood cellularity and granulocyte-macrophage progenitor numbers. When both partners of CBA/N and CBA/T6T6 mice in parabiosis had been treated with phenylhydrazine, spleens and femoral bone marrow of both mice were shown to contain partner-derived CFU-f. These data suggest that circulating CFU-f represent a stromal cell population which can migrate into haemopoietic organs.
| Original language | English |
|---|---|
| Pages (from-to) | 589-95 |
| Number of pages | 7 |
| Journal | Cell and tissue kinetics |
| Volume | 18 |
| Issue number | 6 |
| Publication status | Published - Nov 1985 |
Keywords
- Anemia, Hemolytic
- Animals
- Bone Marrow
- Bone Marrow Cells
- Fibroblasts
- Hematopoietic Stem Cells
- Kinetics
- Mice
- Mice, Inbred CBA
- Mice, Inbred Strains
- Phenylhydrazines
- Spleen