TY - JOUR
T1 - MicroRNA Expression in Myocardial Tissue and Plasma of Patients with End-Stage Heart Failure during LVAD Support
T2 - Comparison of Continuous and Pulsatile Devices
AU - Lok, Sjoukje I.
AU - de Jonge, Nicolaas
AU - van Kuik, Joyce
AU - van Geffen, Ankie J. P.
AU - Huibers, Manon M. H.
AU - van der Weide, Petra
AU - Siera, Erica
AU - Winkens, Bjorn
AU - Doevendans, Pieter A.
AU - de Weger, Roel A.
AU - Martins, Paula A. da Costa
PY - 2015/10/2
Y1 - 2015/10/2
N2 - AimPulsatile flow left ventricular assist devices (pf-LVADs) are being replaced by continuous flow LVADs (cf-LVADs) in patients with end-stage heart failure (HF). MicroRNAs (miRs) play an important role in the onset and progression of HF. Our aim was to analyze cardiac miR expression patterns associated with each type of device, to analyze differences in the regulation of the induced cardiac changes.Methods and ResultsTwenty-six miRs were selected (based on micro-array data and literature studies) and validated in myocardial tissue before and after pf- (n = 17) and cf-LVAD (n = 17) support. Of these, 5 miRs displayed a similar expression pattern among the devices (miR-129*, miR-146a, miR-155, miR-221, miR-222), whereas others only changed significantly during pf-LVAD (miR-let-7i, miR-21, miR-378, miR-378*) or cf-LVAD support (miR-137). In addition, 4 miRs were investigated in plasma of cf-LVAD supported patients (n = 18) and healthy controls (n = 10). Circulating miR-21 decreased at 1, 3, and 6 months after LVAD implantation. MiR-146a, miR-221 and miR-222 showed a fluctuating time pattern post-LVAD.ConclusionOur data show a different miR expression pattern after LVAD support, suggesting that differentially expressed miRs are partially responsible for the cardiac morphological and functional changes observed after support. However, the miR expression patterns do not seem to significantly differ between pf- and cf-LVAD implying that most cardiac changes or clinical outcomes specific to each device do not relate to differences in miR expression levels.
AB - AimPulsatile flow left ventricular assist devices (pf-LVADs) are being replaced by continuous flow LVADs (cf-LVADs) in patients with end-stage heart failure (HF). MicroRNAs (miRs) play an important role in the onset and progression of HF. Our aim was to analyze cardiac miR expression patterns associated with each type of device, to analyze differences in the regulation of the induced cardiac changes.Methods and ResultsTwenty-six miRs were selected (based on micro-array data and literature studies) and validated in myocardial tissue before and after pf- (n = 17) and cf-LVAD (n = 17) support. Of these, 5 miRs displayed a similar expression pattern among the devices (miR-129*, miR-146a, miR-155, miR-221, miR-222), whereas others only changed significantly during pf-LVAD (miR-let-7i, miR-21, miR-378, miR-378*) or cf-LVAD support (miR-137). In addition, 4 miRs were investigated in plasma of cf-LVAD supported patients (n = 18) and healthy controls (n = 10). Circulating miR-21 decreased at 1, 3, and 6 months after LVAD implantation. MiR-146a, miR-221 and miR-222 showed a fluctuating time pattern post-LVAD.ConclusionOur data show a different miR expression pattern after LVAD support, suggesting that differentially expressed miRs are partially responsible for the cardiac morphological and functional changes observed after support. However, the miR expression patterns do not seem to significantly differ between pf- and cf-LVAD implying that most cardiac changes or clinical outcomes specific to each device do not relate to differences in miR expression levels.
KW - VENTRICULAR ASSIST DEVICE
KW - MECHANICAL CIRCULATORY SUPPORT
KW - FAILING HUMAN HEART
KW - CARDIAC-HYPERTROPHY
KW - CONTINUOUS-FLOW
KW - SIGNATURE
KW - RECOVERY
KW - RNA
KW - CARDIOMYOPATHY
KW - REVERSAL
U2 - 10.1371/journal.pone.0136404
DO - 10.1371/journal.pone.0136404
M3 - Article
C2 - 26430739
SN - 1932-6203
VL - 10
JO - PLoS ONE [E]
JF - PLoS ONE [E]
IS - 10
M1 - 0136404
ER -