Method for prediction of an immune response against mismatched human leukocyte antigens

The invention relates to a method for prediction of an immune response against human leukocyte antigens (HLA) after transplantation, said method comprising HLA-typing of the donor and/or donor material and recipient to determine HLA-mismatches and determination of the number of predicted indirectly recognized HLA epitopes (PIRCHES). The invention therefore provides methods for selecting and/or screening donor material for allogeneic transplantation, for example for selecting donor material with permissible mismatches from mismatched unrelated donors. In preferred embodiments the method relates to pre-transplantation prediction of an unwanted alloreactivity that could occur after transplantation of hematopoietic stem cells, cord blood, kidneys and/or other cells, tissues or organs.