TY - JOUR
T1 - Melt Electrospinning Writing of Poly-Hydroxymethylglycolide-co-ε-Caprolactone-Based Scaffolds for Cardiac Tissue Engineering
AU - Castilho, Miguel
AU - Feyen, Dries
AU - Flandes-Iparraguirre, María
AU - Hochleitner, Gernot
AU - Groll, Jürgen
AU - Doevendans, Pieter A.F.
AU - Vermonden, Tina
AU - Ito, Keita
AU - Sluijter, Joost P.G.
AU - Malda, Jos
N1 - Funding Information:
M.C. and D.F. contributed equally to this work. The authors gratefully thank the strategic alliance University Medical Center Utrecht–Eindhoven University of Technology and the European Research Council (ERC) (consolidator grants 3D-JOINT, (#647426) and Design-2Heal (#617989)) for the financial support. The authors acknowledge the support from Innovation and the Netherlands CardioVascular Research Initiative (CVON): The Dutch Heart Foundation, Dutch Federation of University Medical Centers, the Netherlands Organization for Health Research and Development, and the Royal Netherlands Academy of Science, and the support of the European Commission, Marie Curie Individual Fellowships Program (708459). Furthermore, the authors also thank Prof. P. Dalton, supported by a Hofvijverkring Fellowship, for the discussions and valuable suggestions and they greatly appreciate the assistance of C. Metz with the biological testing.
Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/9
Y1 - 2017/9
N2 - Current limitations in cardiac tissue engineering revolve around the inability to fully recapitulate the structural organization and mechanical environment of native cardiac tissue. This study aims at developing organized ultrafine fiber scaffolds with improved biocompatibility and architecture in comparison to the traditional fiber scaffolds obtained by solution electrospinning. This is achieved by combining the additive manufacturing of a hydroxyl-functionalized polyester, (poly(hydroxymethylglycolide-co-ε-caprolactone) (pHMGCL), with melt electrospinning writing (MEW). The use of pHMGCL with MEW vastly improves the cellular response to the mechanical anisotropy. Cardiac progenitor cells (CPCs) are able to align more efficiently along the preferential direction of the melt electrospun pHMGCL fiber scaffolds in comparison to electrospun poly(ε-caprolactone)-based scaffolds. Overall, this study describes for the first time that highly ordered microfiber (4.0-7.0 μm) scaffolds based on pHMGCL can be reproducibly generated with MEW and that these scaffolds can support and guide the growth of CPCs and thereby potentially enhance their therapeutic potential.
AB - Current limitations in cardiac tissue engineering revolve around the inability to fully recapitulate the structural organization and mechanical environment of native cardiac tissue. This study aims at developing organized ultrafine fiber scaffolds with improved biocompatibility and architecture in comparison to the traditional fiber scaffolds obtained by solution electrospinning. This is achieved by combining the additive manufacturing of a hydroxyl-functionalized polyester, (poly(hydroxymethylglycolide-co-ε-caprolactone) (pHMGCL), with melt electrospinning writing (MEW). The use of pHMGCL with MEW vastly improves the cellular response to the mechanical anisotropy. Cardiac progenitor cells (CPCs) are able to align more efficiently along the preferential direction of the melt electrospun pHMGCL fiber scaffolds in comparison to electrospun poly(ε-caprolactone)-based scaffolds. Overall, this study describes for the first time that highly ordered microfiber (4.0-7.0 μm) scaffolds based on pHMGCL can be reproducibly generated with MEW and that these scaffolds can support and guide the growth of CPCs and thereby potentially enhance their therapeutic potential.
KW - Cardiac tissue engineering
KW - Cell orientation
KW - Functional scaffolds
KW - Melt electrospinning writing
KW - Polymer processing
UR - http://www.scopus.com/inward/record.url?scp=85023163924&partnerID=8YFLogxK
U2 - 10.1002/adhm.201700311
DO - 10.1002/adhm.201700311
M3 - Article
C2 - 28699224
AN - SCOPUS:85023163924
SN - 2192-2640
VL - 6
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
IS - 18
M1 - 1700311
ER -