TY - JOUR
T1 - Mechanical alterations of the hippocampus in the APP/PS1 Alzheimer's disease mouse model
AU - Antonovaite, Nelda
AU - Hulshof, Lianne A
AU - Huffels, Christiaan F M
AU - Hol, Elly M
AU - Wadman, Wytse J
AU - Iannuzzi, Davide
N1 - Funding Information:
The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Program (FP/2007?2013)/ERC grant agreement no. [615170] and the Alzheimer Society in the Netherlands (Alzheimer Nederland WE.03-2017-04). The authors further thank M. Marrese for fruitful discussions, E. Paardekam for manufacturing indentation probes, and T. Smit for providing brain tissue slices and support in the lab.
Funding Information:
The research leading to these results has received funding from the European Research Council under the European Union’s Seventh Framework Program (FP/2007–2013)/ERC grant agreement no. [615170] and the Alzheimer Society in the Netherlands (Alzheimer Nederland WE.03-2017-04). The authors further thank M. Marrese for fruitful discussions, E. Paardekam for manufacturing indentation probes, and T. Smit for providing brain tissue slices and support in the lab.
Publisher Copyright:
© 2021
PY - 2021/10
Y1 - 2021/10
N2 - There is increasing evidence of altered tissue mechanics in neurodegeneration. However, due to difficulties in mechanical testing procedures and the complexity of the brain, there is still little consensus on the role of mechanics in the onset and progression of neurodegenerative diseases. In the case of Alzheimer's disease (AD), magnetic resonance elastography (MRE) studies have indicated viscoelastic differences in the brain tissue of AD patients and healthy controls. However, there is a lack of viscoelastic data from contact mechanical testing at higher spatial resolution. Therefore, we report viscoelastic maps of the hippocampus obtained by a dynamic indentation on brain slices from the APP/PS1 mouse model where individual brain regions are resolved. A comparison of viscoelastic parameters shows that regions in the hippocampus of the APP/PS1 mice are significantly stiffer than wild-type (WT) mice and have increased viscous dissipation. Furthermore, indentation mapping at the cellular scale directly on the plaques and their surroundings did not show local alterations in stiffness although overall mechanical heterogeneity of the tissue was high (SD∼40%).
AB - There is increasing evidence of altered tissue mechanics in neurodegeneration. However, due to difficulties in mechanical testing procedures and the complexity of the brain, there is still little consensus on the role of mechanics in the onset and progression of neurodegenerative diseases. In the case of Alzheimer's disease (AD), magnetic resonance elastography (MRE) studies have indicated viscoelastic differences in the brain tissue of AD patients and healthy controls. However, there is a lack of viscoelastic data from contact mechanical testing at higher spatial resolution. Therefore, we report viscoelastic maps of the hippocampus obtained by a dynamic indentation on brain slices from the APP/PS1 mouse model where individual brain regions are resolved. A comparison of viscoelastic parameters shows that regions in the hippocampus of the APP/PS1 mice are significantly stiffer than wild-type (WT) mice and have increased viscous dissipation. Furthermore, indentation mapping at the cellular scale directly on the plaques and their surroundings did not show local alterations in stiffness although overall mechanical heterogeneity of the tissue was high (SD∼40%).
KW - Alzheimer's disease
KW - Biomechanical testing
KW - Brain mechanics
KW - Viscoelasticity
UR - http://www.scopus.com/inward/record.url?scp=85109716054&partnerID=8YFLogxK
U2 - 10.1016/j.jmbbm.2021.104697
DO - 10.1016/j.jmbbm.2021.104697
M3 - Article
C2 - 34271406
SN - 1878-0180
VL - 122
SP - 1
EP - 9
JO - Journal of the mechanical behavior of biomedical materials
JF - Journal of the mechanical behavior of biomedical materials
M1 - 104697
ER -