Measuring rates of ATP synthesis

Matthew J. Bird; Silvia Radenkovic; Pieter Vermeersch; David Cassiman

doi:10.1007/978-1-4939-8769-6_7

Measuring rates of ATP synthesis

Matthew J. Bird^*, Silvia Radenkovic, Pieter Vermeersch, David Cassiman

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

Here, we offer you a high-throughput assay to measure the ATP synthesis capacity in cells or isolated mitochondria. More specifically, the assay is linked to the mitochondrial’ electron transport chain components of your interest being either through complex I (with or without a linkage to pyruvate dehydrogenase activity), through complex II, or through the electron transport flavoprotein and complex I (β-oxidation of fatty acids).

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	97-107
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-8769-6_7
Publication status	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1862
ISSN (Print)	1064-3745

Keywords

ATP synthesis
Bioenergetics
Mitochondria
Oxidative phosphorylation
Oxidative phosphorylation (OXPHOS)
β-oxidation

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10.1007/978-1-4939-8769-6_7

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AU - Radenkovic, Silvia

AU - Vermeersch, Pieter

AU - Cassiman, David

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PY - 2019

Y1 - 2019

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KW - Bioenergetics

KW - Mitochondria

KW - Oxidative phosphorylation

KW - Oxidative phosphorylation (OXPHOS)

KW - β-oxidation

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T3 - Methods in Molecular Biology

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PB - Humana Press Inc.

ER -

Measuring rates of ATP synthesis

Abstract

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