Measurement of antibody-mediated binding of human polymorphonuclear leukocytes to HSV-1 infected anchorage fibroblasts

Kok P M Van Kessel; Henny J. Van Kats-Renaud; Jos A G Van Strijp; Maarten R. Visser; Jan Verhoef

doi:10.1016/0022-1759(86)90057-8

Measurement of antibody-mediated binding of human polymorphonuclear leukocytes to HSV-1 infected anchorage fibroblasts

Kok P M Van Kessel^*
, Henny J. Van Kats-Renaud
, Jos A G Van Strijp
, Maarten R. Visser
, Jan Verhoef

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

A method for the quantitation of effector cell binding to anchorage fibroblast monolayers infected with HSV-1 is described. Human peripheral blood polymorphonuclear leukocytes (PMN) as effector cells were labeled with chromium-51. Fetal human lung fibroblasts were grown to confluency in microtiter plates, infected with HSV-1 and loaded with anti-HSV antibody. The amount of radiolabeled PMN adhering to the monolayer was determined after appropriate incubation and washings. The effector binding assay was shown to be dependent on specific anti-HSV antibodied, antibody concentration, HSV viral expression, and inoculation time. This assay system is especially useful for the evaluation of effector to target cell conjugate formation when applied to anchorage target cells.

Original language	English
Pages (from-to)	101-107
Number of pages	7
Journal	Journal of Immunological Methods
Volume	88
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(86)90057-8
Publication status	Published - 3 Apr 1986

Keywords

Antibody-dependent cell-mediated cytotoxicity
Binding
Herpes simplex virus-infected target
Polymorphonuclear leukocyte

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10.1016/0022-1759(86)90057-8

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title = "Measurement of antibody-mediated binding of human polymorphonuclear leukocytes to HSV-1 infected anchorage fibroblasts",

abstract = "A method for the quantitation of effector cell binding to anchorage fibroblast monolayers infected with HSV-1 is described. Human peripheral blood polymorphonuclear leukocytes (PMN) as effector cells were labeled with chromium-51. Fetal human lung fibroblasts were grown to confluency in microtiter plates, infected with HSV-1 and loaded with anti-HSV antibody. The amount of radiolabeled PMN adhering to the monolayer was determined after appropriate incubation and washings. The effector binding assay was shown to be dependent on specific anti-HSV antibodied, antibody concentration, HSV viral expression, and inoculation time. This assay system is especially useful for the evaluation of effector to target cell conjugate formation when applied to anchorage target cells.",

keywords = "Antibody-dependent cell-mediated cytotoxicity, Binding, Herpes simplex virus-infected target, Polymorphonuclear leukocyte",

author = "\{Van Kessel\}, \{Kok P M\} and \{Van Kats-Renaud\}, \{Henny J.\} and \{Van Strijp\}, \{Jos A G\} and Visser, \{Maarten R.\} and Jan Verhoef",

year = "1986",

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doi = "10.1016/0022-1759(86)90057-8",

language = "English",

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T1 - Measurement of antibody-mediated binding of human polymorphonuclear leukocytes to HSV-1 infected anchorage fibroblasts

AU - Van Kessel, Kok P M

AU - Van Kats-Renaud, Henny J.

AU - Van Strijp, Jos A G

AU - Visser, Maarten R.

AU - Verhoef, Jan

PY - 1986/4/3

Y1 - 1986/4/3

N2 - A method for the quantitation of effector cell binding to anchorage fibroblast monolayers infected with HSV-1 is described. Human peripheral blood polymorphonuclear leukocytes (PMN) as effector cells were labeled with chromium-51. Fetal human lung fibroblasts were grown to confluency in microtiter plates, infected with HSV-1 and loaded with anti-HSV antibody. The amount of radiolabeled PMN adhering to the monolayer was determined after appropriate incubation and washings. The effector binding assay was shown to be dependent on specific anti-HSV antibodied, antibody concentration, HSV viral expression, and inoculation time. This assay system is especially useful for the evaluation of effector to target cell conjugate formation when applied to anchorage target cells.

AB - A method for the quantitation of effector cell binding to anchorage fibroblast monolayers infected with HSV-1 is described. Human peripheral blood polymorphonuclear leukocytes (PMN) as effector cells were labeled with chromium-51. Fetal human lung fibroblasts were grown to confluency in microtiter plates, infected with HSV-1 and loaded with anti-HSV antibody. The amount of radiolabeled PMN adhering to the monolayer was determined after appropriate incubation and washings. The effector binding assay was shown to be dependent on specific anti-HSV antibodied, antibody concentration, HSV viral expression, and inoculation time. This assay system is especially useful for the evaluation of effector to target cell conjugate formation when applied to anchorage target cells.

KW - Antibody-dependent cell-mediated cytotoxicity

KW - Binding

KW - Herpes simplex virus-infected target

KW - Polymorphonuclear leukocyte

UR - https://www.scopus.com/pages/publications/0022653230

U2 - 10.1016/0022-1759(86)90057-8

DO - 10.1016/0022-1759(86)90057-8

M3 - Article

C2 - 3007623

AN - SCOPUS:0022653230

SN - 0022-1759

VL - 88

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EP - 107

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1

ER -

Measurement of antibody-mediated binding of human polymorphonuclear leukocytes to HSV-1 infected anchorage fibroblasts

Abstract

Keywords

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