Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes

Renee G.C. Maas, Soah Lee, Magdalena Harakalova, Christian J.B. Snijders Blok, William R. Goodyer, Jesper Hjortnaes, Pieter A.F.M. Doevendans, Linda W. Van Laake, Jolanda van der Velden, Folkert W. Asselbergs, Joseph C. Wu, Joost P.G. Sluijter, Sean M. Wu*, Jan W. Buikema

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)
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Abstract

Since the discovery of human induced pluripotent stem cells (hiPSCs), numerous strategies have been established to efficiently derive cardiomyocytes from hiPSCs (hiPSC-CMs). Here, we describe a cost-effective strategy for the subsequent massive expansion (>250-fold) of high-purity hiPSC-CMs relying on two aspects: removal of cell-cell contacts and small-molecule inhibition with CHIR99021. The protocol maintains CM functionality, allows cryopreservation, and the cells can be used in downstream assays such as disease modeling, drug and toxicity screening, and cell therapy. For complete details on the use and execution of this protocol, please refer to Buikema (2020).

Original languageEnglish
Article number100334
JournalSTAR protocols
Volume2
Issue number1
DOIs
Publication statusPublished - 19 Mar 2021

Keywords

  • Cell culture
  • Cell differentiation
  • Stem cells

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