Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images

M. Veta; A. Huisman; M. A. Viergever; P. J. Van Diest; J. P.W. Pluim

doi:10.1109/ISBI.2011.5872483

Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images

M. Veta^*, A. Huisman, M. A. Viergever, P. J. Van Diest, J. P.W. Pluim

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

25 Citations (Scopus)

Abstract

In this paper we present an unsupervised automatic method for segmentation of nuclei in H&E stained breast cancer biopsy images. Colour deconvolution and morphological operations are used to preprocess the images in order to remove irrelevant structures. Candidate nuclei locations, obtained with the fast radial symmetry transform, act as markers for a marker-controlled watershed segmentation. Watershed regions that are unlikely to represent nuclei are removed in the postprocessing stage. The proposed algorithm is evaluated on a number of images that are representative of the diversity in pathology in this type of tissue. The method shows good performance in terms of the number of segmented nuclei and segmentation accuracy.

Original language	English
Title of host publication	2011 8th IEEE International Symposium on Biomedical Imaging
Subtitle of host publication	From Nano to Macro, ISBI'11
Pages	618-621
Number of pages	4
DOIs	https://doi.org/10.1109/ISBI.2011.5872483
Publication status	Published - 2 Nov 2011
Event	2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11 - Chicago, IL, United States Duration: 30 Mar 2011 → 2 Apr 2011

Conference

Conference	2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11
Country/Territory	United States
City	Chicago, IL
Period	30/03/11 → 2/04/11

Keywords

breast cancer
histology images
Marker-controlled watershed
nuclei segmentation

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10.1109/ISBI.2011.5872483

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title = "Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images",

abstract = "In this paper we present an unsupervised automatic method for segmentation of nuclei in H&E stained breast cancer biopsy images. Colour deconvolution and morphological operations are used to preprocess the images in order to remove irrelevant structures. Candidate nuclei locations, obtained with the fast radial symmetry transform, act as markers for a marker-controlled watershed segmentation. Watershed regions that are unlikely to represent nuclei are removed in the postprocessing stage. The proposed algorithm is evaluated on a number of images that are representative of the diversity in pathology in this type of tissue. The method shows good performance in terms of the number of segmented nuclei and segmentation accuracy.",

keywords = "breast cancer, histology images, Marker-controlled watershed, nuclei segmentation",

author = "M. Veta and A. Huisman and Viergever, {M. A.} and {Van Diest}, {P. J.} and Pluim, {J. P.W.}",

year = "2011",

month = nov,

day = "2",

doi = "10.1109/ISBI.2011.5872483",

language = "English",

isbn = "9781424441280",

pages = "618--621",

booktitle = "2011 8th IEEE International Symposium on Biomedical Imaging",

note = "2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11 ; Conference date: 30-03-2011 Through 02-04-2011",

}

Veta, M, Huisman, A, Viergever, MA , Van Diest, PJ & Pluim, JPW 2011, Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images. in 2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11., 5872483, pp. 618-621, 2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11, Chicago, IL, United States, 30/03/11. https://doi.org/10.1109/ISBI.2011.5872483

Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images. / Veta, M.; Huisman, A.; Viergever, M. A. et al.
2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11. 2011. p. 618-621 5872483.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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T1 - Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images

AU - Veta, M.

AU - Huisman, A.

AU - Viergever, M. A.

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AB - In this paper we present an unsupervised automatic method for segmentation of nuclei in H&E stained breast cancer biopsy images. Colour deconvolution and morphological operations are used to preprocess the images in order to remove irrelevant structures. Candidate nuclei locations, obtained with the fast radial symmetry transform, act as markers for a marker-controlled watershed segmentation. Watershed regions that are unlikely to represent nuclei are removed in the postprocessing stage. The proposed algorithm is evaluated on a number of images that are representative of the diversity in pathology in this type of tissue. The method shows good performance in terms of the number of segmented nuclei and segmentation accuracy.

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KW - histology images

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Y2 - 30 March 2011 through 2 April 2011

ER -

Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images

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