Mapping the Global Chromatin Connectivity Network for Sox2 Function in Neural Stem Cell Maintenance

Jessica A. Bertolini, Rebecca Favaro, Yanfen Zhu, Miriam Pagin, Chew Yee Ngan, Chee Hong Wong, Harianto Tjong, Marit W. Vermunt, Ben Martynoga, Cristiana Barone, Jessica Mariani, Marcos Julián Cardozo, Noemi Tabanera, Federico Zambelli, Sara Mercurio, Sergio Ottolenghi, Paul Robson, Menno P. Creyghton, Paola Bovolenta, Giulio PavesiFrancois Guillemot, Silvia K. Nicolis*, Chia Lin Wei

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    6 Citations (Scopus)

    Abstract

    Bertolini et al. report that long-range chromatin interactions in neural stem cells (NSCs) are enriched in Sox2-bound enhancers; in Sox2-deleted NSCs, interactions are reduced. Genes downregulated in Sox2-deleted cells are enriched in interactions with enhancers normally Sox2-bound. Overexpression of Socs3, a gene downregulated in mutant NSCs, rescues long-term NSC self-renewal.

    Original languageEnglish
    Pages (from-to)462-476.e6
    JournalCell Stem Cell
    Volume24
    Issue number3
    DOIs
    Publication statusPublished - 7 Mar 2019

    Keywords

    • ChIA-PET
    • chromatin connectivity
    • neural stem cells
    • SOX2
    • transcription factors

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