Maintenance of cellular vitamin B 6 levels and mitochondrial oxidative function depend on pyridoxal 5′-phosphate homeostasis protein

Jolita Ciapaite*, Carlo W T van Roermund, Marjolein Bosma, Johan Gerrits, Sander M Houten, Lodewijk IJlst, Hans R Waterham, Clara D M van Karnebeek, Ronald J A Wanders, Fried J T Zwartkruis, Judith J Jans, Nanda M Verhoeven-Duif

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Recently, biallelic variants in PLPBP coding for pyridoxal 5'-phosphate homeostasis protein (PLPHP) were identified as a novel cause of early-onset vitamin B 6-dependent epilepsy. The molecular function and precise role of PLPHP in vitamin B 6 metabolism are not well understood. To address these questions, we used PLPHP-deficient patient skin fibroblasts and HEK293 cells and YBL036C (PLPHP ortholog)-deficient yeast. We showed that independent of extracellular B 6 vitamer type (pyridoxine, pyridoxamine, or pyridoxal), intracellular pyridoxal 5'-phosphate (PLP) was lower in PLPHP-deficient fibroblasts and HEK293 cells than controls. Culturing cells with pyridoxine or pyridoxamine led to the concentration-dependent accumulation of pyridoxine 5'-phosphate and pyridoxamine 5'-phosphate (PMP), respectively, suggesting insufficient pyridox(am)ine 5'-phosphate oxidase activity. Experiments utilizing 13C 4-pyridoxine confirmed lower pyridox(am)ine 5'-phosphate oxidase activity and revealed increased fractional turnovers of PLP and pyridoxal, indicating increased PLP hydrolysis to pyridoxal in PLPHP-deficient cells. This effect could be partly counteracted by inactivation of pyridoxal phosphatase. PLPHP deficiency had a distinct effect on mitochondrial PLP and PMP, suggesting impaired activity of mitochondrial transaminases. Moreover, in YBL036C-deficient yeast, PLP was depleted and PMP accumulated only with carbon sources requiring mitochondrial metabolism. Lactate and pyruvate accumulation along with the decrease of tricarboxylic acid cycle intermediates downstream of α-ketoglutarate suggested impaired mitochondrial oxidative metabolism in PLPHP-deficient HEK293 cells. We hypothesize that impaired activity of mitochondrial transaminases may contribute to this depletion. Taken together, our study provides new insights into the pathomechanisms of PLPBP deficiency and reinforces the link between PLPHP function, vitamin B 6 metabolism, and mitochondrial oxidative metabolism.

Original languageEnglish
Article number105047
Number of pages15
JournalThe Journal of biological chemistry
Volume299
Issue number9
Early online date13 Jul 2023
DOIs
Publication statusPublished - Sept 2023

Keywords

  • mitochondrial dysfunction
  • pyridox(am)ine 5′-phosphate oxidase (PNPO)
  • pyridoxal 5′-phosphate homeostasis protein PLPHP (PROSC)
  • transaminases
  • vitamin B metabolism
  • α-ketoglutarate

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