TY - JOUR
T1 - Low fetal fraction in cell-free DNA testing is associated with adverse pregnancy outcome
T2 - Analysis of a subcohort of the TRIDENT-2 study
AU - Becking, Ellis C.
AU - Wirjosoekarto, Soetinah A.M.
AU - Scheffer, Peter G.
AU - Huiskes, Julia V.M.
AU - Remmelink, Marinka J.
AU - Sistermans, Erik A.
AU - Bax, Caroline J.
AU - Weiss, Janneke M.
AU - Henneman, Lidewij
AU - Bekker, Mireille N.
N1 - Funding Information:
Soetinah A. M. Wirjosoekarto, Ellis C. Becking, Eril A. Sistermans, Lidewij Henneman, and Mireille N. Bekker are all involved in the TRIDENT‐2 study (Dutch NIPT Consortium) supported by a grant from the Netherlands Organization for Health Research and Development (ZonMw, No. 543002001).
Publisher Copyright:
© 2021 The Authors. Prenatal Diagnosis published by John Wiley & Sons Ltd.
PY - 2021/9
Y1 - 2021/9
N2 - Objectives: To assess the association between low fetal fraction (FF) in prenatal cell-free DNA (cfDNA) testing and adverse pregnancy outcomes. Methods: We conducted a retrospective cohort study of participants of the TRIDENT-2 study (Dutch nationwide government-supported study offering cfDNA screening for fetal aneuploidies) who received a failed test result due to low FF (<4%) between April 2017 until February 2018. Outcome measures included pregnancy-induced hypertension (PIH), pre-eclampsia (PE), small for gestational age neonates (SGA), spontaneous preterm birth (sPTB), gestational diabetes mellitus (GDM), chromosomal aberrations, and congenital structural anomalies. Results: Test failure due to low FF occurred in 295 women (1.12% of tests performed). Information regarding pregnancy outcomes was available for 96.3% of these women. The incidence of PIH, PE, SGA, sPTB, and GDM was 11.2%, 4.1%, 7.3%, 5.1%, and 14.8%, respectively. The prevalence of chromosomal aberrations and congenital structural anomalies was 1.4% and 4.1%, respectively. Incidences of PIH, PE ≥ 34 weeks of gestation, GDM, and prevalence of aneuploidy and congenital structural anomalies were higher in women with low FF compared to the general Dutch obstetric population. Conclusion: Low FF is associated with adverse pregnancy outcomes. The value of FF in the prediction of these outcomes needs to be further established.
AB - Objectives: To assess the association between low fetal fraction (FF) in prenatal cell-free DNA (cfDNA) testing and adverse pregnancy outcomes. Methods: We conducted a retrospective cohort study of participants of the TRIDENT-2 study (Dutch nationwide government-supported study offering cfDNA screening for fetal aneuploidies) who received a failed test result due to low FF (<4%) between April 2017 until February 2018. Outcome measures included pregnancy-induced hypertension (PIH), pre-eclampsia (PE), small for gestational age neonates (SGA), spontaneous preterm birth (sPTB), gestational diabetes mellitus (GDM), chromosomal aberrations, and congenital structural anomalies. Results: Test failure due to low FF occurred in 295 women (1.12% of tests performed). Information regarding pregnancy outcomes was available for 96.3% of these women. The incidence of PIH, PE, SGA, sPTB, and GDM was 11.2%, 4.1%, 7.3%, 5.1%, and 14.8%, respectively. The prevalence of chromosomal aberrations and congenital structural anomalies was 1.4% and 4.1%, respectively. Incidences of PIH, PE ≥ 34 weeks of gestation, GDM, and prevalence of aneuploidy and congenital structural anomalies were higher in women with low FF compared to the general Dutch obstetric population. Conclusion: Low FF is associated with adverse pregnancy outcomes. The value of FF in the prediction of these outcomes needs to be further established.
UR - http://www.scopus.com/inward/record.url?scp=85114520351&partnerID=8YFLogxK
U2 - 10.1002/pd.6034
DO - 10.1002/pd.6034
M3 - Article
C2 - 34426993
AN - SCOPUS:85114520351
SN - 0197-3851
VL - 41
SP - 1296
EP - 1304
JO - Prenatal Diagnosis
JF - Prenatal Diagnosis
IS - 10
ER -