Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium

Janetta Top; Antoni P.A. Hendrickx; Marleen T.J. van Ampting; Kees van Limpt; Jan Knol; Denise van de Kamer; Johanna C. Braat; Marco Viveen; Malbert R. Rogers; Hans Kemperman; Rob J.L. Willems; Fernanda L. Paganelli

doi:10.1002/mbo3.936

Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium

Janetta Top^*
, Antoni P.A. Hendrickx
, Marleen T.J. van Ampting
, Kees van Limpt
, Jan Knol
, Denise van de Kamer
, Johanna C. Braat
, Marco Viveen
, Malbert R. Rogers
, Hans Kemperman
, Rob J.L. Willems
, Fernanda L. Paganelli

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The aim of this study was to determine whether dietary intervention influenced luminal Ca²⁺ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi-synthetic food AIN93 were compared to mice fed AIN93-low calcium (LC). Administration of AIN93-LC resulted in lower luminal Ca²⁺ levels independent of the presence of E. faecium. Furthermore, E. faecium gut colonization was reduced in mice fed AIN93-LC based on culture, and which was in concordance with a reduction of Enterococcaceae in microbiota analysis. In conclusion, diet intervention might be a strategy for controlling gut colonization of E. faecium, an important opportunistic nosocomial pathogen.

Original language	English
Article number	e936
Number of pages	6
Journal	MicrobiologyOpen
Volume	8
DOIs	https://doi.org/10.1002/mbo3.936
Publication status	Published - 1 Dec 2019

Keywords

Enterococcus faecium
gut colonization
low-calcium diet
mouse model

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title = "Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium",

abstract = "The aim of this study was to determine whether dietary intervention influenced luminal Ca2+ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi-synthetic food AIN93 were compared to mice fed AIN93-low calcium (LC). Administration of AIN93-LC resulted in lower luminal Ca2+ levels independent of the presence of E. faecium. Furthermore, E. faecium gut colonization was reduced in mice fed AIN93-LC based on culture, and which was in concordance with a reduction of Enterococcaceae in microbiota analysis. In conclusion, diet intervention might be a strategy for controlling gut colonization of E. faecium, an important opportunistic nosocomial pathogen.",

keywords = "Enterococcus faecium, gut colonization, low-calcium diet, mouse model",

author = "Janetta Top and Hendrickx, \{Antoni P.A.\} and \{van Ampting\}, \{Marleen T.J.\} and \{van Limpt\}, Kees and Jan Knol and \{van de Kamer\}, Denise and Braat, \{Johanna C.\} and Marco Viveen and Rogers, \{Malbert R.\} and Hans Kemperman and Willems, \{Rob J.L.\} and Paganelli, \{Fernanda L.\}",

note = "Publisher Copyright: {\textcopyright} 2019 The Authors. MicrobiologyOpen published by John Wiley \& Sons Ltd.",

year = "2019",

month = dec,

day = "1",

doi = "10.1002/mbo3.936",

language = "English",

volume = "8",

journal = "MicrobiologyOpen",

publisher = "John Wiley \& Sons Inc.",

}

TY - JOUR

T1 - Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium

AU - Top, Janetta

AU - Hendrickx, Antoni P.A.

AU - van Ampting, Marleen T.J.

AU - van Limpt, Kees

AU - Knol, Jan

AU - van de Kamer, Denise

AU - Braat, Johanna C.

AU - Viveen, Marco

AU - Rogers, Malbert R.

AU - Kemperman, Hans

AU - Willems, Rob J.L.

AU - Paganelli, Fernanda L.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - The aim of this study was to determine whether dietary intervention influenced luminal Ca2+ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi-synthetic food AIN93 were compared to mice fed AIN93-low calcium (LC). Administration of AIN93-LC resulted in lower luminal Ca2+ levels independent of the presence of E. faecium. Furthermore, E. faecium gut colonization was reduced in mice fed AIN93-LC based on culture, and which was in concordance with a reduction of Enterococcaceae in microbiota analysis. In conclusion, diet intervention might be a strategy for controlling gut colonization of E. faecium, an important opportunistic nosocomial pathogen.

AB - The aim of this study was to determine whether dietary intervention influenced luminal Ca2+ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi-synthetic food AIN93 were compared to mice fed AIN93-low calcium (LC). Administration of AIN93-LC resulted in lower luminal Ca2+ levels independent of the presence of E. faecium. Furthermore, E. faecium gut colonization was reduced in mice fed AIN93-LC based on culture, and which was in concordance with a reduction of Enterococcaceae in microbiota analysis. In conclusion, diet intervention might be a strategy for controlling gut colonization of E. faecium, an important opportunistic nosocomial pathogen.

KW - Enterococcus faecium

KW - gut colonization

KW - low-calcium diet

KW - mouse model

UR - https://www.scopus.com/pages/publications/85073979912

U2 - 10.1002/mbo3.936

DO - 10.1002/mbo3.936

M3 - Article

C2 - 31568701

AN - SCOPUS:85073979912

VL - 8

JO - MicrobiologyOpen

JF - MicrobiologyOpen

M1 - e936

ER -

Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium

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Keywords

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