Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium

Janetta Top; Antoni P.A. Hendrickx; Marleen T.J. van Ampting; Kees van Limpt; Jan Knol; Denise van de Kamer; Johanna C. Braat; Marco Viveen; Malbert R. Rogers; Hans Kemperman; Rob J.L. Willems; Fernanda L. Paganelli

doi:10.1002/mbo3.936

Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium

Janetta Top^*, Antoni P.A. Hendrickx, Marleen T.J. van Ampting, Kees van Limpt, Jan Knol, Denise van de Kamer, Johanna C. Braat, Marco Viveen, Malbert R. Rogers, Hans Kemperman, Rob J.L. Willems, Fernanda L. Paganelli

^*Corresponding author for this work

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Abstract

The aim of this study was to determine whether dietary intervention influenced luminal Ca²⁺ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi-synthetic food AIN93 were compared to mice fed AIN93-low calcium (LC). Administration of AIN93-LC resulted in lower luminal Ca²⁺ levels independent of the presence of E. faecium. Furthermore, E. faecium gut colonization was reduced in mice fed AIN93-LC based on culture, and which was in concordance with a reduction of Enterococcaceae in microbiota analysis. In conclusion, diet intervention might be a strategy for controlling gut colonization of E. faecium, an important opportunistic nosocomial pathogen.

Original language	English
Article number	e936
Number of pages	6
Journal	MicrobiologyOpen
Volume	8
DOIs	https://doi.org/10.1002/mbo3.936
Publication status	Published - 1 Dec 2019

Keywords

Enterococcus faecium
gut colonization
low-calcium diet
mouse model

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title = "Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium",

abstract = "The aim of this study was to determine whether dietary intervention influenced luminal Ca2+ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi-synthetic food AIN93 were compared to mice fed AIN93-low calcium (LC). Administration of AIN93-LC resulted in lower luminal Ca2+ levels independent of the presence of E. faecium. Furthermore, E. faecium gut colonization was reduced in mice fed AIN93-LC based on culture, and which was in concordance with a reduction of Enterococcaceae in microbiota analysis. In conclusion, diet intervention might be a strategy for controlling gut colonization of E. faecium, an important opportunistic nosocomial pathogen.",

keywords = "Enterococcus faecium, gut colonization, low-calcium diet, mouse model",

author = "Janetta Top and Hendrickx, {Antoni P.A.} and {van Ampting}, {Marleen T.J.} and {van Limpt}, Kees and Jan Knol and {van de Kamer}, Denise and Braat, {Johanna C.} and Marco Viveen and Rogers, {Malbert R.} and Hans Kemperman and Willems, {Rob J.L.} and Paganelli, {Fernanda L.}",

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month = dec,

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doi = "10.1002/mbo3.936",

language = "English",

volume = "8",

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TY - JOUR

T1 - Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium

AU - Top, Janetta

AU - Hendrickx, Antoni P.A.

AU - van Ampting, Marleen T.J.

AU - van Limpt, Kees

AU - Knol, Jan

AU - van de Kamer, Denise

AU - Braat, Johanna C.

AU - Viveen, Marco

AU - Rogers, Malbert R.

AU - Kemperman, Hans

AU - Willems, Rob J.L.

AU - Paganelli, Fernanda L.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - The aim of this study was to determine whether dietary intervention influenced luminal Ca2+ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi-synthetic food AIN93 were compared to mice fed AIN93-low calcium (LC). Administration of AIN93-LC resulted in lower luminal Ca2+ levels independent of the presence of E. faecium. Furthermore, E. faecium gut colonization was reduced in mice fed AIN93-LC based on culture, and which was in concordance with a reduction of Enterococcaceae in microbiota analysis. In conclusion, diet intervention might be a strategy for controlling gut colonization of E. faecium, an important opportunistic nosocomial pathogen.

AB - The aim of this study was to determine whether dietary intervention influenced luminal Ca2+ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi-synthetic food AIN93 were compared to mice fed AIN93-low calcium (LC). Administration of AIN93-LC resulted in lower luminal Ca2+ levels independent of the presence of E. faecium. Furthermore, E. faecium gut colonization was reduced in mice fed AIN93-LC based on culture, and which was in concordance with a reduction of Enterococcaceae in microbiota analysis. In conclusion, diet intervention might be a strategy for controlling gut colonization of E. faecium, an important opportunistic nosocomial pathogen.

KW - Enterococcus faecium

KW - gut colonization

KW - low-calcium diet

KW - mouse model

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VL - 8

JO - MicrobiologyOpen

JF - MicrobiologyOpen

M1 - e936

ER -

Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium

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Keywords

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