TY - JOUR
T1 - Loss of microbial topography between oral and nasopharyngeal microbiota and development of respiratory infections early in life
AU - Man, Wing Ho
AU - Clerc, Melanie
AU - de Steenhuijsen Piters, Wouter A.A.
AU - van Houten, Marlies A.
AU - Chu, Mei Ling J.N.
AU - Kool, Jolanda
AU - Keijser, Bart J.F.
AU - Sanders, Elisabeth A.M.
AU - Bogaert, Debby
N1 - Funding Information:
*These authors contributed equally to this work. ‡Present address: Department of Pulmonary Medicine, St. Antonius Hospital, Nieuwegein, the Netherlands. This work was supported in part by The Netherlands Organization for Health Research and Development (ZonMW; grant 91209010), The Netherlands Organization for Scientific Research (NWO-VIDI; grant 91715359), Wilhelmina Children’s Hospital and Spaarne Gasthuis Hoofddorp intramural funds, a Chief Scientist Office/NHS Research Scotland Scottish Senior Clinical Fellowship award (SCAF/16/03), the University of Edinburgh, and Top Consortia for Knowledge and Innovation (Agri and Food; TKI-AF-12190).
Publisher Copyright:
Copyright © 2019 by the American Thoracic Society
PY - 2019/9/15
Y1 - 2019/9/15
N2 - Rationale: The respiratory microbiota is increasingly being appreciated as an important mediator in the susceptibility to childhood respiratory tract infections (RTIs). Pathogens are presumed to originate from the nasopharyngeal ecosystem. Objectives: To investigate the association between early life respiratory microbiota and development of childhood RTIs. Methods: In a prospective birth cohort (Microbiome Utrecht Infant Study: MUIS), we characterized the oral microbiota longitudinally from birth until 6 months of age of 112 infants (nine regular samples/subject) and compared them with nasopharyngeal microbiota using 16S-rRNA–based sequencing. We also characterized oral and nasopharynx samples during RTI episodes in the first half year of life. Measurements and Main Results: Oral microbiota were driven mostly by feeding type, followed by age, mode of delivery, and season of sampling. In contrast to our previously published associations between nasopharyngeal microbiota development and susceptibility to RTIs, oral microbiota development was not directly associated with susceptibility to RTI development. However, we did observe an influx of oral taxa, such as Neisseria lactamica, Streptococcus, Prevotella nanceiensis, Fusobacterium, and Janthinobacterium lividum, in the nasopharyngeal microbiota before and during RTIs, which was accompanied by reduced presence and abundance of Corynebacterium, Dolosigranulum, and Moraxella spp. Moreover, this phenomenon was accompanied by reduced niche differentiation indicating loss of ecological topography preceding confirmed RTIs. This loss of ecological topography was further augmented by start of daycare, and linked to consecutive development of symptomatic infections. Conclusions: Together, our results link the loss of topography to subsequent development of RTI episodes. This may lead to new insights for prevention of RTIs and antibiotic use in childhood.
AB - Rationale: The respiratory microbiota is increasingly being appreciated as an important mediator in the susceptibility to childhood respiratory tract infections (RTIs). Pathogens are presumed to originate from the nasopharyngeal ecosystem. Objectives: To investigate the association between early life respiratory microbiota and development of childhood RTIs. Methods: In a prospective birth cohort (Microbiome Utrecht Infant Study: MUIS), we characterized the oral microbiota longitudinally from birth until 6 months of age of 112 infants (nine regular samples/subject) and compared them with nasopharyngeal microbiota using 16S-rRNA–based sequencing. We also characterized oral and nasopharynx samples during RTI episodes in the first half year of life. Measurements and Main Results: Oral microbiota were driven mostly by feeding type, followed by age, mode of delivery, and season of sampling. In contrast to our previously published associations between nasopharyngeal microbiota development and susceptibility to RTIs, oral microbiota development was not directly associated with susceptibility to RTI development. However, we did observe an influx of oral taxa, such as Neisseria lactamica, Streptococcus, Prevotella nanceiensis, Fusobacterium, and Janthinobacterium lividum, in the nasopharyngeal microbiota before and during RTIs, which was accompanied by reduced presence and abundance of Corynebacterium, Dolosigranulum, and Moraxella spp. Moreover, this phenomenon was accompanied by reduced niche differentiation indicating loss of ecological topography preceding confirmed RTIs. This loss of ecological topography was further augmented by start of daycare, and linked to consecutive development of symptomatic infections. Conclusions: Together, our results link the loss of topography to subsequent development of RTI episodes. This may lead to new insights for prevention of RTIs and antibiotic use in childhood.
KW - Child
KW - Development
KW - Respiratory microbiota
KW - Respiratory tract infections
KW - Risk factors
UR - https://www.scopus.com/pages/publications/85072160302
U2 - 10.1164/rccm.201810-1993OC
DO - 10.1164/rccm.201810-1993OC
M3 - Article
C2 - 30883192
AN - SCOPUS:85072160302
SN - 1073-449X
VL - 200
SP - 760
EP - 770
JO - American Journal of Respiratory and Critical Care Medicine
JF - American Journal of Respiratory and Critical Care Medicine
IS - 6
ER -