Lnterleukin-5 potentiates sulfidopeptide leukotriene production by human eosinophils

A. J.M. Van Oosterhout; A. Van Der Poel; F. P. Nijkamp; D. Roos; L. Koenderman

doi:10.1155/S0962935194000098

Lnterleukin-5 potentiates sulfidopeptide leukotriene production by human eosinophils

A. J.M. Van Oosterhout^*, A. Van Der Poel, F. P. Nijkamp, D. Roos, L. Koenderman

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

20 Citations (Scopus)

Abstract

Interleukin-5 (IL-5) has been shown to be a selective eosinophil growth and differentiation factor. In the present study, the effect of recombinant human IL-5 on human eosinophil sulfidopeptide leukotriene production was investigated. IL-5 did not affect leukotriene synthesis in unstimulated eosinophils. However, IL-5 potentiated leukotriene synthesis by eosinophils stimulated with serum treated zymosan (STZ) or the calcium ionophore A23187 by 69% and 135%, respectively. The priming effect of IL-5 was dose dependent, with significant stimulation occurring at 1 000 U/ml for STZ and 100-1 000 U/ml for A23187. Pre-incubation with IL-5 did not increase leukotriene synthesis further.

Original language	English
Pages (from-to)	53-55
Number of pages	3
Journal	Mediators of inflammation
Volume	3
Issue number	1
DOIs	https://doi.org/10.1155/S0962935194000098
Publication status	Published - 1 Jan 1994

Keywords

Eosinophil
Interleukin-5
Leukotriene

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title = "Lnterleukin-5 potentiates sulfidopeptide leukotriene production by human eosinophils",

abstract = "Interleukin-5 (IL-5) has been shown to be a selective eosinophil growth and differentiation factor. In the present study, the effect of recombinant human IL-5 on human eosinophil sulfidopeptide leukotriene production was investigated. IL-5 did not affect leukotriene synthesis in unstimulated eosinophils. However, IL-5 potentiated leukotriene synthesis by eosinophils stimulated with serum treated zymosan (STZ) or the calcium ionophore A23187 by 69% and 135%, respectively. The priming effect of IL-5 was dose dependent, with significant stimulation occurring at 1 000 U/ml for STZ and 100-1 000 U/ml for A23187. Pre-incubation with IL-5 did not increase leukotriene synthesis further.",

keywords = "Eosinophil, Interleukin-5, Leukotriene",

author = "{Van Oosterhout}, {A. J.M.} and {Van Der Poel}, A. and Nijkamp, {F. P.} and D. Roos and L. Koenderman",

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T1 - Lnterleukin-5 potentiates sulfidopeptide leukotriene production by human eosinophils

AU - Van Oosterhout, A. J.M.

AU - Van Der Poel, A.

AU - Nijkamp, F. P.

AU - Roos, D.

AU - Koenderman, L.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Interleukin-5 (IL-5) has been shown to be a selective eosinophil growth and differentiation factor. In the present study, the effect of recombinant human IL-5 on human eosinophil sulfidopeptide leukotriene production was investigated. IL-5 did not affect leukotriene synthesis in unstimulated eosinophils. However, IL-5 potentiated leukotriene synthesis by eosinophils stimulated with serum treated zymosan (STZ) or the calcium ionophore A23187 by 69% and 135%, respectively. The priming effect of IL-5 was dose dependent, with significant stimulation occurring at 1 000 U/ml for STZ and 100-1 000 U/ml for A23187. Pre-incubation with IL-5 did not increase leukotriene synthesis further.

AB - Interleukin-5 (IL-5) has been shown to be a selective eosinophil growth and differentiation factor. In the present study, the effect of recombinant human IL-5 on human eosinophil sulfidopeptide leukotriene production was investigated. IL-5 did not affect leukotriene synthesis in unstimulated eosinophils. However, IL-5 potentiated leukotriene synthesis by eosinophils stimulated with serum treated zymosan (STZ) or the calcium ionophore A23187 by 69% and 135%, respectively. The priming effect of IL-5 was dose dependent, with significant stimulation occurring at 1 000 U/ml for STZ and 100-1 000 U/ml for A23187. Pre-incubation with IL-5 did not increase leukotriene synthesis further.

KW - Eosinophil

KW - Interleukin-5

KW - Leukotriene

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DO - 10.1155/S0962935194000098

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JO - Mediators of inflammation

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Lnterleukin-5 potentiates sulfidopeptide leukotriene production by human eosinophils

Abstract

Keywords

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