Live imaging of mRNA using RNA-stabilized fluorogenic proteins

Jiahui Wu; Sara Zaccara; Deepak Khuperkar; Hyaeyeong Kim; Marvin E. Tanenbaum; Samie R. Jaffrey

doi:10.1038/s41592-019-0531-7

Live imaging of mRNA using RNA-stabilized fluorogenic proteins

Jiahui Wu, Sara Zaccara, Deepak Khuperkar, Hyaeyeong Kim, Marvin E. Tanenbaum, Samie R. Jaffrey^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA–protein complexes that enable live imaging of mRNA in living cells.

Original language	English
Pages (from-to)	862-865
Number of pages	4
Journal	Nature Methods
Volume	16
Issue number	9
DOIs	https://doi.org/10.1038/s41592-019-0531-7
Publication status	Published - 1 Sept 2019

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abstract = "Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA–protein complexes that enable live imaging of mRNA in living cells.",

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AU - Tanenbaum, Marvin E.

AU - Jaffrey, Samie R.

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AB - Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA–protein complexes that enable live imaging of mRNA in living cells.

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Live imaging of mRNA using RNA-stabilized fluorogenic proteins

Abstract

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