Live imaging of cell division in 3D stem-cell organoid cultures

Ana C.F. Bolhaqueiro, Richard H. van Jaarsveld, Bas Ponsioen, René M. Overmeer, Hugo J. Snippert, Geert J.P.L. Kops*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Examining cell behavior in its correct tissue context is a major challenge in cell biology. The recent development of mammalian stem cell-based organoid cultures offers exciting opportunities to visualize dynamic cellular events in a 3D tissue-like setting. We describe here an approach for live imaging of cell division processes in intestinal organoid cultures derived from human and mouse adult stem cells. These approaches can be extended to the analysis of cellular events in diseased tissue, such as patient-derived tumor organoids.

Original languageEnglish
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages91-106
Number of pages16
Volume145
ISBN (Print)9780128141427
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Cell Biology
Volume145
ISSN (Print)0091-679X

Keywords

  • Aneuploidy
  • Chromosome
  • Mitosis
  • Organoid
  • Stem cell
  • Tumor

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